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首页> 外文期刊>African Journal of Biotechnology >In vitro mass propagation of an epiphytic orchid,Dendrobium primulinum Lindl. through shoot tip culture
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In vitro mass propagation of an epiphytic orchid,Dendrobium primulinum Lindl. through shoot tip culture

机译：外膜兰花，石斛植物的体外繁殖。通过拍摄提示文化

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摘要

The present study develops a protocol for rapid?in vitro?micropropagation of a?critically endangered and floriculturally most important epiphytic orchid,?Dendrobium primulinum?Lindl. through?the?culture of small shoot tip explants (0.3 to 0.5mm) derived from?in vitro?grown seedlings. The shoot tip explants cultured on solidified Murashige and Skoog?(MS) basal medium and MS medium alone or supplemented with combination of various concentrations of growth regulators; α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP), produced shoots and multiple shoots. The maximum numbers of rootless healthy shoots were observed on MS medium fortified with BAP 1.5 mgl-1?with an average value of 4.5 shoots per culture where shoot multiplication was initiated after 5 weeks of culture of shoot tip. Among the different tested combination, MS medium with BAP (1.5 mg l-1) and NAA (0.5 mg l-1) were most effective for the shoot multiplication. MS medium supplemented with various concentrations of rooting hormones viz. NAA, IAA and IBA showed positive response in development of roots, except NAA 0.5 mgl-1. The rooting was observed after 3 weeks of culture of shoot tip. The various concentrations of IAA and IBA were found to be effective hormone for rooting of?D. primulinum?in comparison to NAA. The best rooting response was observed on MS medium with exogenous supply of IAA 0.5 mgl-1. The well developed?in vitro?rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2:1. Nearly 70% of plantlets survived.
机译：本研究开发了一种快速的方案，体外呢？危重濒危和鲜花养殖最重要的果实兰花的微扑衰减，？叶片原素瘤？LINDL。通过？α培养小枝粒子的外植体（0.3〜0.5mm）衍生自含有β生长的幼苗。茎尖外植体在凝固的Murashige和Skoog上培养？（MS）基础培养基和MS培养基单独或补充各种浓度的生长调节剂; α-萘乙酸（NAA）和6-苄基氨基嘌呤（BAP），产生的芽和多次芽。在与BAP 1.5 MGL-1强化的MS培养基上观察到无无能健康芽的最大数量？平均值为每种培养物的4.5芽，在射击尖端5周后开始拍摄倍增。在不同的测试组合中，具有烤盘（1.5mg L-1）和NAA（0.5mg L-1）的MS培养基对于芽倍增最有效。 MS培养基补充有各种根茎激素ZIZ。 NAA，IAA和IBA在ROOTS开发中显示出积极的反应，除NAA 0.5 MGL-1外。在3周的芽尖培养后观察到生根。发现各种浓度的IAA和IBA是有效的激素，用于生根？D. Primulinum？与NAA相比。在MS培养基上观察到最佳生根响应，具有外源供应IAA 0.5 MGL-1。发育良好的含量良好？在含有共匹配和SpHagnum苔藓的灌封混合物中成功地硬化了植物的植株，其比例为2：1。近70％的植物幸存下来。

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