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首页> 外文期刊>African Journal of Biotechnology >Sporangiospore-yeast transformation of Mucor circinelloides: Ionic circulation, chemical potential, sodium influx rate and morphogenesis
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Sporangiospore-yeast transformation of Mucor circinelloides: Ionic circulation, chemical potential, sodium influx rate and morphogenesis

机译：Mucor循环素的SporangioSpore-yeast转化：离子循环，化学潜力，流入率和形态发生

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Measurement of intracellular ion concentration during sporangiospores-yeast transformation of?Mucor circinelloides?Tieghem in K+- mediated (0.90 to 1.10 g/l) and Na+-modulated (0.05 to 0.20 g/l) multiionic broths, pH 4.5, temperature 20°C, showed that (a), transmembrane ion flux was continuous during the growth period (b), Na+efflux varied concurrently with K+?influx and vice versa (c), induced morphologies varied: growth spheres, enterothallic-, holothallic-, holoblastic- conidia, septate hyphae with vesicular conidial head groups, and terminal budding yeast cells but protoplasts and granular particles were transient (d), time-course plots of log transformed optical density measurements gave sigmoid growth pattern principally at 1.0 g/l K+?and 0.10 g/l Na+?incorporation where the induced morphology was mainly terminal budding yeast but primary and secondary growth optima occurred at the other levels and these were thought to result from determinate thallic growth: septate hyphae transformed into enterothallic conidia; cytosolic nucleates/granular particles could be extruded after thallic cell wall lyses or conidial burst, which then collapsed; the granular units thereafter became protoplasts and could assume the yeast morphology (e), ionic profiles showed correlation with growth phases: lag phase was accompanied with Na+?efflux with simultaneous influx of K+; exponential growth phase- rapid influx of Na+?and concurrent efflux of K+; stationary phase- rapid efflux of Na+?with simultaneous, albeit slow intracellular accumulation of K+?. It was strongly suggested that ion fluxes were mediated by the P-type, plasma-membrane localized, cation ATPases. This study shows?that morphological expression was correlated with the intracellular ion contents. The chemical potentials (m) were calculated at the pre- logarithmic growth phases and these influenced cytosolic nucleation. As the magnitude of?m?increased, cytosolic nucleates were less readily formed. Apparently, a balance of Na+?and K+, deputed as critical concentration (cc), was required for cytosolic nucleation. But transition from protoplasts to the yeast form was thought to be dependent on the magnitude of the Na+?influx rate (x).?We?conclude that highly functional cooperativity existed between?m,?x, as well as?cc, for successful transformation of sporangiospores to terminal budding yeast cells.
机译：测量旋转孢子孢子素 - 酵母转化期间的细胞内离子浓度的测量粘膜循环蛋白β - 介导的静电（0.90-1.10g / L）和Na + - 常规（0.05至0.20g / L）偏相传肉汤，pH 4.5，温度20°C显示（a），在生长期间（b）期间，跨膜离子通量是连续的，Na + efflux与k + k +流入，反之亦然（c），诱导的形态变化：生长球，肠溶液 - ，holothallic-，holoplic - Conidia，用囊泡分析头组的癸酸盐菌丝，和末端芽孢粒细胞，但原生质体和颗粒状颗粒是瞬态（d），对数转化的光密度测量的时间手术图主要以1.0g / l k +的六种生长模式给予血管生长模式。和0.10g / l na +？诱导形态主要是终端芽酵母的掺入，但初级和二次生长最佳发生在其他水平上，这些水平被认为是测定的铊生长：豆腐菌根形成肠内属的分类;细胞骨核酸/颗粒状颗粒可以在铊细胞壁裂解物或分枝突发后挤出，然后分枝裂缝;粒状单位之后变成原生质体，可以假设酵母形态（e），离子谱表现出与生长阶段的相关性：滞后阶段伴随着Na +Δfeflux，同时吹入K +; Na +的指数增长阶段 - 快速流入K +的k +;静止相 - 快速的Na +？具有同时的，尽管k +慢的细胞内积聚慢。强烈建议离子助熔剂由p型，血浆膜局部化，阳离子酶介导。本研究表明？形态学表达与细胞内离子含量相关。在对数性生长阶段计算化学电位（M），这些影响的细胞溶质成核。随着ωm的幅度增加，细胞溶质成核较小。显然，细胞溶质成核需要Na +和K +的平衡，偏向于临界浓度（CC）。但从原生质体过渡到酵母形式的过渡被认为是依赖于Na +？流入速率（x）的幅度。？我们结论是，在ΔM，？x和xcc之间存在高度的功能合作，以及成功孢子囊孢子转化为末端芽孢芽细胞。

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