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首页> 外文期刊>African Journal of Biotechnology >Characterization and stability of d-amino acid oxidase and catalase within the permeabilized recombinant Pichia pastoris cells
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Characterization and stability of d-amino acid oxidase and catalase within the permeabilized recombinant Pichia pastoris cells

机译：渗透性重组粒子牧草细胞中D-氨基酸氧化酶和过氧化氢酶的表征及稳定性

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摘要

An effort was devoted to the detailed characterization and stability analysis of?d-amino acid oxidase (DAAO) and catalase (CAT) in the permeabilized cells, involved in a summary of our previous works. It was observed that the?culture conditions, the permeabilization treatments, the enzyme leakage and pH value of solution except the enzyme expression levels, displayed a significant effect on the enzyme stability. About 96% of the CAT activity in the cetyltrimethylammonium bromide (CTAB)-permeabilized cells was inhibited when treated?with sodium azide (0.8?m, 30 min), while no visible effect on the activity and stability of DAAO was detected. Though,almost 100% of the CAT activity in the CTAB-permeabilized cells could be eliminatedby the alkali treatment (pH 11.5, 30 min), both the activity and stability of DAAO were slightly impaired. The half-life of DAAO could be up to 15.5 days by treating the CTAB-permeabilized cells with 1% glutaraldehyde (GLA). Additionally, it was noted that?the gene sources and expression hosts might play an important role on the stability of DAAO and CAT in permeabilized cells.
机译：致力于在渗透细胞中的Δd-氨基酸氧化酶（Daao）和过氧化氢酶（猫）的详细表征和稳定性分析，参与我们以前的作品的概要。观察到α培养条件，渗透处理，溶液的酶泄漏和pH值除酶表达水平外，对酶稳定性显示出显着影响。当处理时，抑制了约96％的甲酰基溴化铵（CTAB） - 甲基化细胞中的猫活性？用叠氮化钠（0.8Ωm，30分钟），而检测到对Daao的活性和稳定性没有明显的效果。虽然，在碱处理（pH11.5,30分钟）中，近100％的CTAB渗透细胞中的猫活性均略微受损。通过用1％戊二醛（GLA）将CTAB渗透细胞治疗CTAB渗透细胞，DAAO的半衰期最长可达15.5天。另外，有人指出？基因来源和表达宿主可能在透化细胞中的Daao和猫的稳定性上发挥重要作用。

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