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首页> 外文期刊>African Journal of Biotechnology >Cloning of a novel stearoyl-acyl desaturase gene from white ash (Fraxinus americana) and evolution analysis with those from other plants
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Cloning of a novel stearoyl-acyl desaturase gene from white ash (Fraxinus americana) and evolution analysis with those from other plants

机译：来自白灰（Fraxinus Americana）的新型硬脂酰酰基去饱和酶基因和其他植物的克隆分析

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Using?reverse transcription polymerase chain reaction?(RT-PCR)?and rapid amplification of cDNA ends, a new full-length cDNAs of?stearoyl-ACP desturases (SAD)?(FaSAD) was obtained from white ash. Sequence analysis showed that the deduced amino acid sequence of?FaSAD?has high similarity to that of other reported SAD proteins. They are different from each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs.?The analysis?of semi-quantitative?RT-PCR showed that the expression of?SAD?gene had?the highest level in stem and lowest level in leaves. The tertiary structure prediction indicated thatFaSAD?protein should be a compact globular protein. Based on evolution analysis, it was clear that the genes from the same family were approximately clustered into a group, but all genes from woody plants were not clustered into a separate group. In woody plants, it was indicated that all sequences clustered into two major groups and the?FaSAD?from white ash was closely related to the SAD gene fromMacfadyena unguis-cati.
机译：使用α逆转录聚合酶链反应？（RT-PCR）α（RT-PCR）α，CDNA的快速扩增是末端的，是一种新的全长CDNA的αSearoyl-ACP火药（SAD）？（Fasad）是从白灰获得。序列分析表明，推导的氨基酸序列的αfasad？与其他报告的悲伤蛋白质的相似性高。它们通过一些替代，插入和/或缺失彼此不同，涉及单氨基酸残基或术语的分析？半定量的？RT-PCR显示出表达？悲伤？基因有？最高水平叶子中的茎和最低水平。三级结构预测表明，蛋白质应该是紧凑的球状蛋白质。基于进化分析，显然，将来自同一家族的基因大致聚集成一个组，但木质植物的所有基因都不会聚集成单独的组。在木质植物中，表明所有序列都聚集成两个主要群体和？Fasad？来自白灰与来自Macfadyena Unguis-Cati的悲伤基因密切相关。

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