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首页> 外文期刊>African Journal of Biotechnology >A study on the mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzae
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A study on the mechanism of resistance to streptomycin in Xanthomonas oryzae pv. oryzae

机译：Xanthomonas oryzae PV抗链霉素抗性机理的研究。 oryzae.

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11 streptomycin-resistant mutants of?Xanthomonas oryzae?pv.?oryzae?were obtained by streptomycin selection. These mutants could grow at 100 μg ml-1?of streptomycin while the wild-type strain (PXO99) could not grow at 2 μg ml-1.?Specific primers based on the conserved region of?X. oryzae?pv. oryzae?were designed?and used to amplify the gene encoding ribosomal protein S12 (rpsL). Sequencing indicated that the?rpsL gene has 375 bp encoding 125 amino acid residues. One pair of specific primers with restriction enzyme site of?EcoR I?enzyme was designed and used to amplify the?rpsL?gene from streptomycin-resistant strains and the wild-type sensitive strain of?X. oryzae?pv.?oryzae. In all resistant strains, a mutation in which AAG was substituted for AGG (Lys→Arg) occurred either at codon 43 or 88. The mutations situated at codon 43 or 88 in?rpsL gene of streptomycin-resistant?X. oryzae?pv. oryzae?strains could be detected by PCR-RFLP. Two plasmids, pUFRPS and pUFRPX, were constructed by ligating?the?rpsL gene into the cosmid?pUFR034. The plasmids pUFRPS and pUFRPX containing the Lys→Arg mutation of the?rpsL gene conferred streptomycin resistance were transformed into the sensitive wild-type strain by electroporation. Both?transformants,?PS1 and PS2, could grow at 50 μg ml-1of streptomycin. The results strongly suggest that the mutation in?rpsL could result in resistance of?X. oryzae pv. oryzae?to streptomycin.
机译：11致霉素抗链霉素的抗蛋白霉素α-xanthomonas oryzae？pv。？oryzae？通过链霉素选择获得。这些突变体可以在100μgmL-1中生长β-链霉素，而野生型菌株（PXO99）不能在2μgmL-1中生长。基于保守区域的αx的特异性引物。 oryzae？pv。 oryzae？设计了α并用于扩增编码核糖体蛋白S12（RPS1）的基因。测序表明？rps1基因具有375bp编码125氨基酸残基。一种具有限制酶位点的一对特异性引物？EcoRIα的诱导酶网站设计并用于扩增来自链霉素抗性菌株的α1α基因和αx的野生型敏感菌株。 oryzae？pv。？oryzae。在所有抗性菌株中，在密码子43或88处发生Agg（Lys→Arg）取代AAG的突变。位于密码子43或88处的突变抗链霉素抗性Δx的RPSL基因。 oryzae？pv。 oryzae？可以通过PCR-RFLP检测菌株。通过连接αrpsl基因来构建两个质粒，pufrps和pufrpx .PUFR034。通过电穿孔将含有Lys→Arg突变的质粒pufrps和pufrpx赋予链霉素抗性的α1基因抗性抗性。两个？转化体，？ps1和ps2，可以在50μgml-1f霉菌霉素中生长。结果强烈表明突变α1的突变可能导致αx。 oryzae pv。 oryzae？对链霉素。

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