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首页> 外文期刊>African Journal of Biotechnology >Effect of high dose thiamine therapy on activity and molecular aspects of transketolase in Type 2 diabetic patients
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Effect of high dose thiamine therapy on activity and molecular aspects of transketolase in Type 2 diabetic patients

机译：高剂量硫胺素治疗对2型糖尿病患者转铁糖石酶活性和分子方面的影响

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Commonest form of diabetes mellitus is Type 2, treated with oral hypoglycemic agents, which often carry potential adverse effects and do not address the intracellular metabolism of glucose. Thiamine is an essential co-factor for vital subcellular enzymes and has potential to benefit Type 2 diabetics.? This study was therefore designed to investigate the effect of high dose thiamine therapy on the activity and molecular aspects of transketolase in Type 2 diabetic patients.?Over 100 Type 2 microalbuminuric diabetics were enrolled in a randomized, double blind placebo controlled clinical trial for 6 months. Patients were divided into two groups, one treated with 300 mg/day thiamine and the other group was administered placebo for a period of 3 months followed by a 2 month washout period. 50 normal healthy controls participated for baseline estimations only. Transketolase activity of mononuclear cells and erythrocytes were determined. Also q-polymerase chain reaction (PCR) was used to determine expression levels of transketolase gene in mononuclear cells.?All enrolled Type 2 diabetics had > 40% lower mononuclear transketolase activity as compared to healthy individuals. Thiamine therapy for three months resulted in a 65% significant increase in transketolase activity which persisted into washout period. Mononuclear transketolase gene expression was significantly reduced in Type 2 diabetics as compared to normal controls (0.66 fold thiamine group) and (0.89 fold) placebo group). High dose thiamine therapy resulted in highly significant increase (2.86 fold) in expression of transketolase gene in mononuclear cells which was sustained at 2.91 fold after washout period. These results indicate that high dose thiamine therapy improves both transketolase activity and expression in Type 2 diabetic patients with incipient nephropathy.
机译：最常见的糖尿病形式是2型，用口服降血糖治疗，这通常会带来潜在的不利影响，并且不会解决葡萄糖的细胞内代谢。硫胺素是重要的亚细胞酶的必要态因素，并且有可能效益2型糖尿病患者。因此，该研究旨在探讨高剂量硫胺素治疗对2型糖尿病患者转铁糖蛋白酶的活性和分子方面的影响。100型2型微藻糖尿病患者注册随机，双盲安慰剂控制临床试验6个月。患者分为两组，用300毫克/天硫胺化治疗，另一组被施用安慰剂3个月，然后进行2个月的洗涤期。 50个正常健康控制仅参与基线估计。测定单核细胞和红细胞的转蛋白酶活性。还使用Q-聚合酶链反应（PCR）来确定单核细胞中的转酮酶基因的表达水平。与健康个体相比，纳入2型糖尿病患者患有2型糖尿病患者> 40％较低的单核转铁糖蛋白酶活性。硫胺素治疗三个月导致持续到冲洗期的转托酮糖苷酶活性增加了65％。与正常对照（0.66倍硫胺素组）和（0.89倍）安慰剂组相比，单核转发蛋白酶基因表达显着降低2型糖尿病患者。高剂量的硫胺素治疗导致在洗涤期后291倍的单核细胞表达过酮糖苷酶基因表达的高显着增加（2.86倍）。这些结果表明，高剂量的硫胺素治疗改善了2型糖尿病患者的转铁蛋白酶活性和表达初期的肾病。

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