首页> 外文期刊>African Journal of Biotechnology >Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.)
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Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.)

机译:菠萝部分绿叶同源物的克隆与表达分析(Ananas Comosus(L.)Merr。)

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LEAFY-like genes are crucial regulators of flowering in angiosperms. A partial homologue of?LEAFY,?designated as?AcLFY?(Genebank accession no HQ433335), was isolated from pineapple (Ananas comosus?L. cv. Comte de Paris) by PCR. The conserved cDNA fragment of?AcLFY?is 256 bp in length and contained an open reading frame of 248 bp, which encodes 82 amino acids protein. The amino acid alignment showed that?AcLFY?had a high identity with the related fragment ofPhalLFY?in?Phalaenopsis?hybrid cultivar (91%),?LFY?in?Oncidium (90%) and?OrcLFYin?Orchis italica?(88%). RT-PCR analysis showed that the?AcLFY?could be expressed constitutionally. Moreover, it was expressed in the flesh of young fruit and bract at low levels, and was highly expressed in the fruit, stem, petal and sepal.
机译:叶状叶状基因是植物植物中开花的关键调节因素。一种偏爱?叶状物,?指定为?(GeneBank登录没有HQ433335),从菠萝(Ananas Comosus?L.CV.CO.COTEDALIS)分离。保守的cDNA片段?ACLFY?的长度为256bp,含有248bp的开放阅读框,其编码82个氨基酸蛋白。氨基酸对准显示?ACLFY?与噬菌体相关的片段有高度的身份吗?在?植物蛋白?杂交品种(91%),?LFY?在?内胞内(90%)和?Orclfyin?orclfyin?oralica?(88% )。 RT-PCR分析显示?ACLFY?可以在宪法表达。此外,它在幼果的肉中表达,在低水平下苞片,并且在水果,茎,花瓣和萼片中高度表达。

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