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首页> 外文期刊>African Journal of Biotechnology >Influence of plant age on fructan content and fructosyltranserase activity in Agave atrovirens Karw leaves
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Influence of plant age on fructan content and fructosyltranserase activity in Agave atrovirens Karw leaves

机译：植物时代对龙舌兰阿罗韦氏叶片叶片含量和果糖转移酶活性的影响

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摘要

An evaluation of non-structural carbohydrate content, fructan:fructan 1-fructosyltransferase (1-FFT; EC. 2.4.1.100) enzymatic activity and electrophoretic pattern of proteins in?Agave atrovirens?leaves was performed to establish the optimum age for plant harvest. The highest soluble carbohydrate content was observed in 3-year-old leaves, but inulin-type fructan content was highest in 6-year-old leaves. Fructan synthesis in?A. atrovirens?apparently occurs in two sequential reactions; 1-kestose is generated in the first reaction, and inulin-type fructans with degree of polymerization (DP)≥30 are obtained in the second reaction. The 1-FFT enzymatic activity was higher in 6-year-old leaves, coinciding with higher content inulin-type fructans with DP≥29 at this age. This behavior suggests a relationship between 1-FFT enzymatic activity and the concentration of fructans. A monomer was obtained in the 6-year-old leaves (MW ~70 kDa), while in the 3- and 9-year-old leaves, this monomer was associated with three subunits (MW ~50, 17 and 15 kDa) or two subunits (MW ~25 and 17 kDa), respectively. In conclusion, leaf age is a determining factor in the 1-FFT enzymatic activity, which is related to the inulin-type fructans concentration.?Keywords:?Agave, leaves, inulin-type fructans, 1-FFT, age.
机译：对非结构碳水化合物含量的评价，果断：果酱1-果糖转移酶（1-FFT; EC。2.4.1.100）酶活性和蛋白质中的电泳模式在α-龙舌兰阿托烃ααα叶片，以确定植物收获的最佳年龄。在3岁的叶片中观察到最高可溶的碳水化合物含量，但菊粉型叶片含量在6岁的叶子中最高。 Fructan合成在？a。 Atrovirens？显然发生在两个连续反应中;在第一反应中产生1-Kest​​ose，在第二反应中获得具有聚合度（DP）≥30度的菊粉型煎蛋糖。在6岁的叶片中，1-FFT酶活性较高，在此年龄段与DP≥29的含量更高的含含含菊酯型煎蛋糖。这种行为表明1-FFT酶活性和煎锅浓度之间的关系。在6岁的叶片（Mw〜70kDa）中获得单体，而在3岁和9岁的叶子中，该单体与三个亚基（MW〜50,17和15kDa）相关或分别为两个亚基（MW〜25和17 kda）。总之，叶绿性是1-FFT酶活性的决定因素，其与菊粉型Fructans浓度有关。叶片，叶片，菊粉型叶片，1-FFT，年龄。

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