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首页> 外文期刊>African Journal of Biotechnology >High regioselective acetylation of vitamin A precursors using lipase B from Candida antarctica in organic media
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High regioselective acetylation of vitamin A precursors using lipase B from Candida antarctica in organic media

机译：维生素的高区域选择性乙酰化前体使用来自有机培养基中的念珠菌B的脂肪酶B.

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摘要

The effect of different reaction parameters was explored on the acylation of primary hydroxyl group of 1,6-diol by lipase B from?Candida antarctica?catalysis in organic solvent. First, the effect of the organic solvents was investigated, and the highest conversion rate was obtained in?n-hexane. Then, the effect of the acyl donor was studied. Among several reactants, including acetic acid and two different acetates, vinyl acetate gave the best yield. A maximum monoester yield of 98.5% was obtained using vinyl acetate as acyl donor in n-hexane at 50°C. The substrate concentration was 25 mmol/L, while the diol to vinyl acetate molar ratio was 1:3. Substrate concentration had to be limited due to an inhibitory effect on enzyme by the diol that caused a decrease on initial reaction rate. To promote initial reaction rate, excess vinyl acetate was used. Under the optimum conditions, the conversion rate and mono-acylation selectivity were 98.5 and 100%, respectively. The produced monoester was 6.1 mg/ml, and this amount can be further optimized base on the results presented here.?Key word:?Acetylation, regioselectivity, immobilized lipase B, biocatalytic processes, vitamin A precursors.
机译：探讨了不同反应参数的初级羟基酰基通过脂肪酶B通过脂肪酶B的丙酰·南极洲的酰化物质的影响？有机溶剂中的催化。首先，研究了有机溶剂的效果，并在α正己烷中获得最高的转化率。然后，研究了酰基供体的作用。在几种反应物中，包括乙酸和两种不同的乙酸盐，乙酸乙烯酯给出了最佳产率。在50℃下使用乙酸乙烯酯作为酰基供体在N-己烷中获得最大单酯产率98.5％。底物浓度为25mmol / L，而二醇与乙酸乙烯酯摩尔比为1：3。由于对初始反应速率降低的二醇对酶的抑制作用，底物浓度必须受到限制。为了促进初始反应速率，使用过量的乙酸乙烯酯。在最佳条件下，转化率和单酰化选择性分别为98.5和100％。所生产的单酯是6.1mg / ml，可以进一步优化在此提供的结果中的进一步优化。？酮词：α乙酰化，区域选择性，固定化脂肪酶B，生物催化过程，维生素A前体。

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