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首页> 外文期刊>African Journal of Biotechnology >Cloning and sequence analysis of the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale
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Cloning and sequence analysis of the defective in anther dehiscence1 (DAD1) gene fragment of Chinese kale

机译：中国羽衣甘蓝缺乏缺陷的克隆与序列分析

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To clone the?defective in anther dehiscence1?(DAD1) gene fragment of Chinese kale, about 700 bp product was obtained by PCR amplification using Chinese kale genomic DNA as the template and a pair of specific primers designed?according to the conserved sequence of?DAD1?genes of?Arabidopsis thaliana?and?Brassica rapa. The ampli?ed product was ligated into the T vector and sequenced. The results show that the gene fragment was 678 bp long without introns. It shared 89%identity with?the nucleotide sequence of the?DAD1?gene of?A. thaliana?and the sequence identity was as high as 97 to 99% with those of other plants belonging to the same genus as Chinese kale. The amino acid sequence deduced from the nucleotide sequence had 91% identity with that of?A. thaliana. It was shown that the cloned fragment was a part of Chinese kale?DAD1?gene.
机译：为了克隆？在花药Dehiscence1中有缺陷？（DAD1）中国羽衣甘蓝的基因片段，通过PCR扩增使用中国羽衣甘蓝基因组DNA作为模板和设计的一对特异性引物来获得约700bp产物。根据保守的序列DAD1？拟南芥的基因？和？芸苔属rapa。将Ampli-Ed产物连接到T载体中并进行测序。结果表明，基因片段长度为678bp，没有内含子。它共用了89％的同一性？DAD1？基因的核苷酸序列？a。蒂利亚纳？并且序列同一性高达97％至99％，其中其他植物属于中国羽衣甘蓝的其他植物。从核苷酸序列推导的氨基酸序列具有91％的同一性，具有Δa。蒂利亚纳。结果表明，克隆片段是中国羽衣甘蓝的一部分？DAD1？基因。

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