• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Catalase epitopes vaccine design for Helicobacter pylori: A bioinformatics approach
【24h】

Catalase epitopes vaccine design for Helicobacter pylori: A bioinformatics approach

机译：幽门螺杆菌疫苗设计的过氧化氢酶疫苗设计：一种生物信息学方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bioinformatics tools are helpful for epitopes prediction directly from the genomes of pathogens in order to design a vaccine. Epitopes are sub-sequences of proteins (8 to 10 mer peptides) which bind to MHC to interact with the T cell receptors and stimulate immune responses.?Finding a suitable vaccine against?Helicobacter pylori is necessary, because of high prevalence of the infection (25 to 90%). Moreover, this bacteria has been classified as a grade?I carcinogen by WHO since 1994. Catalase, an important enzyme in the virulence of?H. pylori, could be a suitable candidate for vaccine design because it is highly conserved, which is important for the survival of H.?pylori;?it is?expressed in high level and it is?exposed on the surface of the?bacteria.?In this study, we designed epitope-based vaccine for catalasespecific regions of?H. pylori?by means of immunobioinformatic tools.?H. pylori(26695) catalase?has been?compared with human catalase in order to select specific regions. Afterwards, epitopes of catalase were determined by propred software. Among predicted epitopes, three epitopes were selected including, MVNKDVKQTT, VLLQSTWFL and FHPFDVTKI. Three candidates out of 51catalase antigen epitopes had the highest score for reactivating with MHC II MHC in propred software.?The candidate epitopes for vaccine design should be rather a composition of considering epitopes: MVNKDVKQTTKKVLLQSTWFLKKFHPFDVTKI. In this manner, 39 of 51 alleles of MHC class ?? were involved and stimulated T-cell responses. We believe?prediction of catalase epitopes by the immunoinformatics tools would be valuable for developing new immuoprophylatic strategy against?H. pylori?infection.
机译：生物信息学工具有助于直接从病原体的基因组预测到疫苗以设计疫苗。表位是蛋白质的子序列（8至10 MEL肽），其与MHC结合以与T细胞受体相互作用，刺激免疫应答。由于感染的普及率高，需要刺激合适的疫苗，是必要的，因为感染的患病率很高（ 25至90％）。此外，这种细菌已被归类为一种等级？I致癌物质以来，自1994年以来。过氧化氢酶，是毒力的重要酶。幽门螺杆菌可能是疫苗设计的合适候选者，因为它是高度保守的，这对于H.?Pylori的存活是重要的;在高水平的情况下表达，它是？暴露在细菌的表面上。？在这项研究中，我们设计了基于表位的疫苗，用于催化区域的Δh。幽门螺杆呢？通过免疫综合管理工具。幽门螺杆菌（26695）过氧化氢酶？与人过期的酶比较，以便选择特定区域。然后，通过提出的软件确定过氧化氢酶的表位。在预测表位中，选择三种表位，包括MVNKDVKQTT，VLLQSTWFL和FHPFDVTKI。 51℃丙二酸酶抗原表位的三个候选者具有重新激活与MHC II MHC在提出的软件中重新激活的最高分。疫苗设计的候选表位应该是考虑表位的组成：MVNKDVKQTTKKVLLQSTWFLKKFHPFDVTKI。以这种方式，39个中的51个等位基因的MHC类？参与并刺激T细胞反应。我们相信？免疫信息工具的过氧化氢酶表位的预测对于开发新的抗癌策略对抗ΔH。幽门？感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号