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首页> 外文期刊>African Journal of Biotechnology >Complementary DNA-amplified fragment length polymorphism (AFLP-cDNA) analysis of differential gene expression from the xerophyte Ammopiptanthus mongolicus in response to cold, drought and cold together with drought
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Complementary DNA-amplified fragment length polymorphism (AFLP-cDNA) analysis of differential gene expression from the xerophyte Ammopiptanthus mongolicus in response to cold, drought and cold together with drought

机译：互补的DNA扩增片段长度多态性（AFLP-cDNA）分析来自辛辣杏仁胺的差异基因表达与冷，干旱和冷干旱

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In this study, we examine?Ammopiptanthus mongolicus, the only desert ultra-broad-leaved evergreen shrub in Northwest China. During this study,?A. mongolicus?was exposed to drought 20% polyethylene glycol (PEG)), low temperature (4°C) and a combination of drought and low temperature (20% PEG and 4°C). Complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) technology was used to analyze differential transcriptional expression under the aforementioned abiotic stresses. We found that 9 of the studied expressed sequence tags (ESTs) are related to protein modification, 12 ESTs are involved in the control of transcription and translation, 9 ESTs are involved in metabolism, 11 ESTs are related to aging and defense genes, 1 EST plays a role in signal transduction, 3 ESTs are related to cell development, 3 ESTs may play a role in energy production and photosynthesis, 2 ESTs are related to protein transport and 49 ESTs are of unknown significance.
机译：在这项研究中，我们检查了ammopiptanthus mongolicus，这是中国西北部唯一的沙漠超广阔的常绿灌木。在这项研究期间，？a。蒙古斯？暴露于干旱20％聚乙二醇（PEG），低温（4℃）和干旱和低温（20％PEG和4℃）的组合。互补的DNA扩增的片段长度多态性（cDNA-AFLP）技术用于分析上述非生物胁迫下的差异转录表达。我们发现研究的9个表达序列标签（EST）与蛋白质改性有关，12个EST参与转录和翻译的控制，9个EST参与代谢，11个EST与老化和防御基因有关，1 EST在信号转导中发挥作用，3个EST与细胞显影有关，3个EST可能在能量产生和光合作用中发挥作用，2个EST与蛋白质转运有关，49个EST具有未知意义不明显。

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