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首页> 外文期刊>African Journal of Biotechnology >Kinetic investigation on enantioselective hydrolytic resolution of epichlorohydrin by crude epoxide hydrolase from domestic duck liver
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Kinetic investigation on enantioselective hydrolytic resolution of epichlorohydrin by crude epoxide hydrolase from domestic duck liver

机译：鸭肝粗环氧水解酶对表氯醇映选择性水解分辨的动力学研究

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Enantiopure epichlorohydrin is a valuable epoxide intermediate for preparing optically active pharmaceuticals. In this study, a novel epoxide hydrolase prepared from domestic duck liver was used as biocatalyst for producing (S) - epichlorohydrin from its racemates. To characterize the biocatalytic profiles of crude epoxide hydrolase, some effect factors were investigated. The crude epoxide hydrolase has an apparent optimal pH of 8.0 and an optimal temperature of 35°C. Fe2+?and Mn2+?both enhanced the activity of epoxide hydrolase to different degrees. The results of kinetic study revealed that a significant inhibition phenomenon is observed in this reaction process. The apparent kinetic parameters (viz.,?V?max?andKm) and apparent substrate inhibition parameter?Kis?were calculated with linear fitting. The developed production process indicates that the novel epoxide hydrolase from domestic duck liver is a highly efficient biocatalyst for preparing enantiopure epichlorohydrin.
机译：对映异络表氯醇是一种有价值的环氧化物中间体，用于制备光学活性药物。在这项研究中，由家用鸭肝制备的新型环氧化物水解酶被用作生物催化剂，用于从其外消旋体生产 - 表氯醇。为了表征粗氧化物水解酶的生物催化谱，研究了一些影响因素。粗氧化物水解酶具有明显的最佳pH值为8.0，最佳温度为35℃。 Fe2 +？和Mn2 +？同时增强了环氧水解酶的活性至不同程度。动力学研究结果显示在该反应过程中观察到显着的抑制现象。表观动力学参数（viz，？vαmax？andkm）和表观底物抑制参数？KIS？用线性配件计算。开发的生产方法表明，来自家畜鸭肝的新型环氧化物水解酶是一种高效的生物催眠催化剂，用于制备对映对表氯醇。

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