首页> 外文期刊>African Journal of Biotechnology >Effect of salinity on cell growth and -carotene production in Dunaliella sp. isolates from Urmia Lake in northwest of Iran
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Effect of salinity on cell growth and -carotene production in Dunaliella sp. isolates from Urmia Lake in northwest of Iran

机译:盐度对细胞生长和-Carotene SP中的影响。来自伊朗西北乌利米湖的孤立

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Urmia Lake, the second largest hyper-saline lake by area in the world, has fluctuated in salinity over time, but recently, it has reached a maximum of 360 g/l.Dunaliella?is a type of halophile green-orange microalgae especially found in lake and salty fields and is known for its antioxidant activity; because of its ability to create large amount of carotenoids. In the present study,?Dunaliella?sp. isolates from hyper- saline Urmia Lake water were cultured in modified Johnson media and were treated at different salinities (1, 2 and 3 M NaCl), then their cell proliferation rate and β-carotene production were studied. In order to determine the optimal salinity required for the highest β-carotene accumulation, cell count?of?Dunaliella?sp. isolates; total carotenoids and concentration of the β-carotene were determined by direct microscopic counting and spectrophotometry. In the samples with different salinities,?the cell count and the β-carotene content of?Dunaliella?sp. ranged between?0.53 and 2.21x106?cell.ml-1?and 0.2 to 11.4 pg.cell-1, respectively. At the end of the experiments, the maximum?cell content mean?and the highest β-carotene content mean were obtained at 1 and 3 M NaCl concentrations, as 1.68 x 106?cell.ml-1?and 8.94 pg.cell-1, respectively.?Keywords:?Dunaliella, microalgae, Urmia Lake,?chlorophyceae, β-carotene.
机译:乌利亚湖,第二大盐水湖由世界各地,在盐度上波动随着时间的推移,但最近,它已达到360克/ L.dunaliella?是一种嗜烟绿橙色微藻,特别是在湖泊和咸的领域,以其抗氧化活性而闻名;由于其产生大量类胡萝卜素的能力。在本研究中,?Dunaliella?SP。来自盐水荨麻疹水的分离物在改良的Johnson培养基中培养,并在不同的盐水(1,2和3M NaCl)处理,然后研究了它们的细胞增殖率和β-胡萝卜素。为了确定最高β-胡萝卜素积累所需的最佳盐度,细胞计数?Dunaliella?SP。分离;通过直接微观计数和分光光度法测定β-胡萝卜素的总类胡萝卜素和浓度。在具有不同盐度的样品中,?细胞计数和β-胡萝卜素含量?dunaliella?sp。在Δ0.53和2.21x106之间进行范围,分别为0.53和2.21x106?和0.2至11.4 pg.cell-1。在实验结束时,最大?细胞含量意味着?并且在1和3M NaCl浓度下获得最高的β-胡萝卜素含量平均值,如1.68×10 6?Cell.ML-1?和8.94 PG.Cell-1 ,分别为:::::?dunaliella,微藻,荨麻疹,?叶绿素,β-胡萝卜素。

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