• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Cytogenetic, genomic in situ hybridization (GISH) and agronomic characterization of alien addition lines derived from wheat-Psathyrostachys huashanica
【24h】

Cytogenetic, genomic in situ hybridization (GISH) and agronomic characterization of alien addition lines derived from wheat-Psathyrostachys huashanica

机译：细胞遗传学，基因组原位杂交（GISH）和来自小麦 - Psathyrostachys Huashanica的外星添加管线的农艺表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Alien chromosome lines were developed and identified from the?BC1F2?and BC1F3generations?of a wheat-Psathyrostachys huashanica?intergeneric cross. Their agronomic traits were evaluated in the field and their meiotic behaviors and chromosome composition were analyzed by cytogenetic and GISH (genomic?in situhybridization) techniques. Five lines with 2n = 44 and one line with 2n = 46 showed regular meiosis and were cytologically stable. Based?on chromosome pairing, C-banding and GISH analysis, lines 156-4, 160-12, 160-13, 173-2 and 197-16 (2n = 44) were alien disomic addition lines with the addition of one pair of the?P. huashanica?5Ns chromosomes. Line 165-2, which had each pair of the?P. huashanica?chromosomes 3Ns and 5Ns being added, was an alien double addition line. Nine lines with the?P. huashanica?chromosome?1Ns, 2Ns, 4Ns or 5Ns werealien monosomic addition lines.?Robertsonian?translocation was observed in lines 241-2 and 241-10.?Some chromosomal abnormalities were observed such as telosomics, lagging of telosomics and telosomic sister chromatids that moved to one pole?at anaphase I?and asynchronous chromosome separation at telophase II. These results indicated that, the presence of alien chromosomes from?P. huashanica?had influenced the meiosis. Meanwhile,?by comparing the series of wheat-P. huashanica?chromosome addition lines, the gene(s) for awns were mapped on the?P.?huashanica?5Ns?chromosome.
机译：外源染色体株小麦 - 华山新麦草？属间杂交种的开发，并从？BC1F2确定？和BC1F3generations？他们的农艺性状在该领域进行了评估，并对其减数分裂行为和染色体组成是由细胞遗传学和原位杂交分析（基因组？在situhybridization）技术。五线为2n = 44，并用2N = 46一行表明定期减数分裂并且是细胞学稳定。基于？染色体配对，C-带和GISH分析，线156-4，160-12，160-13，173-2和197-16（2N = 44）分别与所述加法1双的外来二体附加系在2 P。华山？5ns的染色体。线165-2，其中有每对的？P的。华山？染色体3NS并添加为5ns，是一个陌生的双附加系。九线与2 P。华山？染色体？为1ns，2NS，4NS或5NS werealien单体附加lines.?Robertsonian?translocation于线241-2和241-10.?Some染色体异常观察到观察到如telosomics，telosomics和telosomic姐妹染色单体落后于移动到一个极？在后期Iα和在末期II异步染色体的分离。这些结果表明，外源染色体从3 P的存在。华山？影响了减数分裂。同时，？通过系列小麦-P的比较。华山？染色体附加系，该基因（一个或多个），用于芒被映射的？P.？华山？5NS？染色体上。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号