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首页> 外文期刊>African Journal of Biotechnology >Identification of genes induced by salt stress from Medicago truncatula L. seedlings
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Identification of genes induced by salt stress from Medicago truncatula L. seedlings

机译：来自Medicago Truncatula L.幼苗盐胁迫诱导的基因的鉴定

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In order to identify genes induced during the salt stress response in barrel medic (Medicago truncatula?L) seedlings, a cDNA library by salt stress was constructed by suppression subtractive hybridization (SSH). Total RNA from 15-day-old seedlings was used as a ‘driver’, and total RNA from seedlings induced by salt was used as a ‘tester’. One hundred and sixty nine clones identified as positive clones by reverse northern dot-blotting resulted in 75 uni-ESTs that comprised of 13 contigs and 62 singletons. Basic Local Alignment Search Tool (BLAST) analysis of deduced protein sequences revealed that 35 expressed sequence tags (ESTs) had identity similar to proteins with known function, while 27 could not be annotated at all. Most of the known function sequences were homologous to genes involved in abiotic stress in plants. Among these protein, citrate synthase, ribulose- 1,5-bisphosphate carboxylase, chloroplast protein, phosphoenolpyruvate carboxylase and chloroplast outer envelope protein are related to photosynthesis; DNA binding/transcription factor, putative AP2/EREBP transcription factor, Cab9 gene, photosystem II polypeptide and calcium-dependent protein kinase play a significant role in signal transduction and transcription regulation; and aldolase and sucrose synthase are interrelated to osmolyte synthesis. Moreover, 5 of the ESTs, similar to genes from other plant species and closely involved in salt stress were isolated from?M. truncatula?L. They are superoxide dimutase (SOD)-1, gene for copper/zinc superoxide dismutase, cysteine protease, Na+/H+?antiporter and salt overly sensitive 2 (SOS2). To further assess the expression level of salt-induced ESTs, real-time polymerase chain reaction (PCR) analysis was employed, and the result showed that these genes have significantly increased expression and probably play an important role in the response of plants to salt stress.
机译：为了鉴定在桶军医（Medicago Truncatulaα1）幼苗中诱导的盐应激反应期间诱导的基因，通过抑制减法杂交（SSH）构建通过盐应激的cDNA文库。将15天幼苗的总RNA用作“驾驶员”，用盐诱导的幼苗的总RNA用作“测试仪”。通过反向北部点印迹鉴定为阳性克隆的一百六十九克隆产生75个Uni-EST，其包含13个葡萄片和62个单身。引用的蛋白质序列的基本局部对准搜索工具（BLAST）分析显示，35种表达的序列标签（EST）具有与具有已知功能的蛋白质相似的同一性，而27根本不能注释。大多数已知的功能序列与参与植物中非生物胁迫的基因同源。在这些蛋白质中，柠檬酸酯合酶，核糖糖 - 1,5-双磷酸羧化酶，叶绿体蛋白，磷酸丙烯酸盐羧化酶和叶绿体外壳蛋白质与光合作用有关; DNA结合/转录因子，推定AP2 / ereBP转录因子，CAB9基因，光系统II多肽和钙依赖性蛋白激酶在信号转导和转录调节中发挥着重要作用;和醛糖酶和蔗糖合成酶与Osmolyte合成相互关联。此外，其中5个与来自其他植物物种的基因类似，与其他植物物种的基因相比，从αm中分离出盐胁迫。 truncatula？l。它们是超氧化物二级酶（SOD）-1，铜/锌超氧化物歧化酶的基因，半胱氨酸蛋白酶，Na + / H +α抗脂剂和盐过敏2（SOS2）。为了进一步评估盐诱导的EST的表达水平，使用实时聚合酶链反应（PCR）分析，结果表明这些基因的表达显着增加，并且可能在植物对盐胁迫下发挥重要作用。

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