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首页> 外文期刊>African Journal of Biotechnology >Comparison of transgenic plant production for bacterial blight resistance in Pakistani local rice (Oryza sativa L.) cultivars
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Comparison of transgenic plant production for bacterial blight resistance in Pakistani local rice (Oryza sativa L.) cultivars

机译：转基因植物生产对巴基斯坦局部稻（Oryza Sativa L.）品种的细菌抗性抗性的比较

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The study was carried out to improve bacterial leaf blight resistance in three rice cultivars (Basmati - 370, DR - 82 and IR - 6) by?Agrobacterium?mediated transformation system. Three week-old scutellum derived calli were infected withAgrobacterium?strain EHA101,?containing binary vector pTCL5 which has Xa 21 gene. Different levels of acetosyringone were tested to enhance transformation efficiency. Acetosyringone at 300 μM showed 56.6%?GUS?expression with 100 and 200 μM acetosyringone showing 13.3 and 30.0%?GUS?expression, respectively. Maximum transformation efficiency was obtained using DR - 82 with calli exposed to 300 μM acetosyringone for 2 min. Direct hygromycin selection with 48 h of co-cultivation was superior to pre-selection in all three cultivars. Transient?GUSexpression was 51.4% while stable?GUS?expression in calli was 18.8%. PCR analysis confirmed the presence of the Xa 21 gene in transformed regenerated plants. Stable varietal transformation efficiency was DR - 82 > Basmati-370 > IR - 6. Resistance of transgenic plants against?Xanthomonas oryzae pathovar oryzaewas evaluated with various strains/isolates at the seedling stage. All PCR positive transgenic plants of DR - 82 and Basmati - 370 were resistant with lesion areas less than 5% of the inoculated leaf area. The tested transgenic plants were resistant to all the indigenous and exotic strains tested due to the broad spectrum protection provided by the Xa 21 gene.
机译：该研究进行了通过α土壤杆菌（Basmati - 370，DR-82和IR-6）在三种水稻品种（Basmati - 370，DR-82和IR-6）中改善细菌叶枯萎病。介导的转化系统。三周龄鳞状衍生的Calli被感染了多久的α101，α菌株EHA101，α含有二元载体PTCl5，其具有XA 21基因。测试了不同水平的乙酰菌酮，以提高转化效率。乙酰菌酮在300μm时显示出56.6％？GUS？表达100和200μM乙酰苯甲丝酮，分别显示13.3和30.0％的αγ表达。使用DR-82获得最大变换效率，愈伤组织暴露于300μm乙酰苯胺酮2分钟。直接潮霉素选择，共培养48小时优于所有三种品种的预选。瞬态？GusExpression稳定为51.4％，稳定？GUS？Calli的表达是18.8％。 PCR分析证实了转化的再生植物中XA 21基因的存在。稳定的品种转化效率是DR - 82> Basmati-370> IR - 6.转基因植物的抗性对抗αXanthomonas Oryzae病毒毒素Oryzaewas在幼苗阶段的各种菌株/分离物评估。所有PCR阳性转基因植物DR-82和Basmati - 370耐受损伤区域的耐损伤区域，小于接种叶区域的5％。由于XA 21基因提供的广谱保护，所测试的转基因植物对所有的土着和异国菌株抵抗。

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