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首页> 外文期刊>African Journal of Biotechnology >Laboratory studies on vegetative regeneration of the gametophyte of Bryopsis hypnoides Lamouroux (Chlorophyta, Bryopsidales)
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Laboratory studies on vegetative regeneration of the gametophyte of Bryopsis hypnoides Lamouroux (Chlorophyta, Bryopsidales)

机译：BRYOPSIS催眠液Lamouroux植物再生的实验室研究（叶绿素，苯染期）

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Vegetative propagation from thallus segments and protoplasts of the gametophyte of?Bryopsis hypnoides?Lamouroux (Chlorophyta, Bryopsidales) was studied in laboratory cultures. Thallus?segments were cultured at 20°C, 20 μmol photons m-2s-1, 12:12 h LD); protoplasts were cultured under various conditions, viz. 15°C, 15 μmol photons m-2?s-1, 10:14 h LD; 20°C, 20 μmol photons m-2?s-1, 12:12 h LD; and 25°C, 25 μmol photons m-2?s-1, 14:10 h LD. Microscope observation revealed that the protoplast used for regeneration was only part of the protoplasm and the regeneration process was complete in 12 h. The survival rate of the segments was 100% and the survival rate of protoplasts was around 15%, regardless of culture conditions. Protoplasts were very stable in culture and were tolerant of unfavorable conditions. Cysts developed at the distal end or middle portion of gametophytic filaments under low illumination (2 - 5 μmol photons m-2?s-1), the key induction factor. Cysts formed several protoplast aggregations inside or the cyst as a whole detached directly from the matrix and all the units were able to develop directly into new gametophytes. Regeneration directly from protoplasts and thallus segments were also discovered in the field. A relatively completed life history of?B. hypnoidesis established with newly discovered propagation methods, namely protoplast regeneration.
机译：在实验室培养物中研究了来自αBryopsis催眠物的菌丝段和原生质体的培养物和原生质体的植物繁殖。在实验室培养中研究了Lamouroux（叶绿素，苔藓糖苷）。 Thallus？段在20℃，20μmol光子M-2S-1,11,12 H LD培养;原生质体在各种条件下培养，viz。 15°C，15μmol光子M-2？S-1,10:14 H LD; 20°C，20μmol光子M-2？S-1,12：12 H LD;和25°C，25μmol光子M-2？S-1,14：10 H LD。显微镜观察显示，用于再生的原生质体仅是原生质的一部分，再生过程在12小时内完成。无论培养条件如何，细分的存活率为100％，原生质体的存活率约为15％。原生质体在培养方面非常稳定，耐受不利条件的耐受性。在低照明（2-5μmol光子M-2→S-1）下，在杂化细丝的远端或中间部分开发的囊肿，关键诱导因子。囊肿形成几种原生质体聚集或囊肿，因为直接从基质中分离，所有单元都能够直接发展到新的配子上。在该领域还发现了直接从原生质体和晶系因的再生。一个相对完成的生活史？b。用新发现的传播方法建立催眠，即原生质体再生。

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