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首页> 外文期刊>African Journal of Biotechnology >Optimizing the production of welan gum by Alcaligenes facalis NX-3 using statistical experiment design
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Optimizing the production of welan gum by Alcaligenes facalis NX-3 using statistical experiment design

机译：使用统计实验设计优化Alcaligenes Facalis NX-3的Welan Gum的生产

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Welan gum is an exopolysaccharide produced by?Alcaligenes?facalis?NX-3. The composition of the medium for the production of welan gum was optimized with a stepwise strategy. Effects of various carbon and nitrogen sources were investigated. Plackett–Burman design was applied to elucidate the key ingredients in the media and the results indicated that the corn starch, cottonseed cake flour have significant effects on welan gum production. Central composite design was employed to search for the optimal concentration of the two key components and the experimental results were fitted to a second-order polynomial model at a 98% level of significance. By solving the regression equation and also by analyzing the response surface contour plots, the relative high welan gum production (22.85 g l-1) was obtained at the optimal medium combinations and fermentation conditions as corn starch 43.6 g l-1, cottonseed cake flour 4.1 g l-1, 16-h-old inoculum of 3% (v v-1) sizes, initial pH 7.0 and temperature 30oC.?These predicted values were also verified by validation experiments.
机译：Welan gum是由αalcaligenes产生的含有脱核酸的果糖？脸部？NX-3。用逐步策略优化了威尔曼胶生产的培养基的组成。研究了各种碳和氮源的影响。 Plackett-Burman设计被应用于阐明培养基的关键成分，结果表明，玉米淀粉，棉籽蛋糕面粉对Welan Gum生产具有显着影响。使用中央复合设计来搜索两个关键部件的最佳浓度，实验结果适用于二阶多项式模型，其显着性的98％。通过求解回归方程以及通过分析响应表面轮廓图，在最佳培养基组合和发酵条件下获得相对高的柔软胶生产（22.85g L-1），为玉米淀粉43.6g l-1，棉籽蛋糕粉4.1g l-1，16-h古造物为3％（v-1）尺寸，初始pH 7.0和温度30℃。通过验证实验还验证了预测值。

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