首页> 外文期刊>African Journal of Biotechnology >No more recalcitrant: Chickpea regeneration and genetic transformation
【24h】

No more recalcitrant: Chickpea regeneration and genetic transformation

机译:没有更顽固的纯粹:鹰嘴豆再生和遗传转化

获取原文
           

摘要

Chickpea production is limited worldwide because of abiotic and biotic stresses. Efforts to overcome these production constraints through traditional breeding are difficult due to limited genetic variation. Novel regeneration is pre-requisite for genetic transformation offers the opportunity to overcome hybridization barriers and introduce novel genes for resistance. Although direct gene transfer via direct DNA transfer has been reported,?Agrobacterium?mediated transformation is the preferred method and standard protocols have been established for the production of transgenic plantlets derived from co-cultivation of embryonic axes. This was soon adopted due to difficulties associated with regeneration of whole plants from callus. Only few reports have been reported using genetic transformation/ transgene(s) against abiotic stress tolerance transgenic chickpea plants. Transgenic chickpea using bacterial?codA?gene tolerance against abiotic stresses have been developed. Chickpea improvement and application of genomics tools to the study of the chickpea genome will be enhanced through the use of genetic transformation.
机译:由于非生物和生物应激,鹰嘴豆产量有限。由于遗传变异有限,通过传统育种克服这些生产限制的努力。新型再生是遗传转化的先决条件,提供了克服杂交障碍的机会,并引入抗性的新基因。虽然已经报道了通过直接DNA转移的直接基因转移,但是农杆菌?介导的转化是优选的方法,已经建立了用于生产衍生自胚胎轴的共培养的转基因植物的标准方案。由于与来自愈伤组织的整个植物的再生有关,这很快就通过了这一点。据报道,使用遗传转化/转基因对非生物胁迫耐受转基因鹰嘴豆植物的遗传转化/转基因仅少数报道。使用细菌的转基因鹰嘴豆乳酸肽瘤?已经开发出对非生物胁迫的基因耐受性。通过使用遗传转化,将提高鸡眼工具对鹰嘴豆基因组的研究的改进和应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号