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首页> 外文期刊>African Journal of Biotechnology >Molecular identification of indigenous bacteria isolated from pesticides heavily contaminated soils
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Molecular identification of indigenous bacteria isolated from pesticides heavily contaminated soils

机译：杀虫剂分离的土着细菌的分子鉴定严重污染的土壤

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This study was carried out to isolate and identify indigenous bacteria associated with pesticides heavily contaminated soil in Sudan. The samples were collected from pesticides contaminated soil in different locations. The predominant bacterial isolates were then purified by subculturing on selective media. Seven isolates were selected based on their capability to degrade pesticides and three of them having the highest performance in biodegradation of frequently reported pesticide contaminants were subject to identification by molecular tools using 16S rDNA gene sequence analysis. Amplification of 16S rDNA gene was done from genomic DNA and sequenced. Construction of phylogenetic tree was done after aligning of multiple sequences. The results reveal that the three bacterial isolates are Bacillus cereus ATCC 14579, Bacillus subtilis subsp. inaquosorum KCTC 13429 and Bacillus safensis FO-36b. This is the first record of pesticides biodegradation by B. safensis FO-36b which can be considered as a novel contribution in this field. Since these strains were found effective and are living naturally in an environment heavily contaminated with pesticides, therefore, they might have potential in removing pesticides from contaminated soils, especially soils of pesticide stores and dumping sites in developing countries.
机译：本研究进行了分离和鉴定与苏丹群体严重受污染的土壤相关的土着细菌。将样品从不同地点的杀虫污染土壤中收集。然后通过在选择性培养基上培养来纯化主要的细菌分离物。根据其降解杀虫剂的能力，并在使用16S RDNA基因序列分析的分子工具的鉴定，基于其降解农药的能力，其中三个具有最高性能的杀虫剂的性能最高。从基因组DNA进行16S rDNA基因的扩增并测序。在对齐多个序列后，在系统发育树的构建进行。结果表明，三种细菌分离株是芽孢杆菌ATCC 14579，枯草芽孢杆菌患者。 INAQUOSORUM KCTC 13429和BACILLUS SAFENSIS FO-36B。这是B. Safensis Fo-36B的第一个杀虫剂生物降解的记录，其可被认为是该领域的新贡献。由于这些菌株被发现有效并且在严重污染杀虫剂的环境中自然生活，因此它们可能有可能在从污染的土壤中去除农药，特别是在发展中国家的农药储存和倾销地点的土壤。

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