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首页> 外文期刊>American Journal of Analytical Chemistry >Simple HPLC Analysis of Hinokitiol in Skin Lotion with Visible Light Detection after Pre-Column Dabsylation
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Simple HPLC Analysis of Hinokitiol in Skin Lotion with Visible Light Detection after Pre-Column Dabsylation

机译：柱子溶解后的皮肤乳液中Hinokitiol的简单HPLC分析

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Hinokitiolis frequently added to personal care products as an antibacterial agent. We previously established an HPLC-UV method for determination of hinokitiol in skin lotion after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole. However, the labeling reagent is expensive, and derivatives of degradation products of parabens, which may be added to skin lotion as general preservatives, interfered with the peak of the hinokitiol derivative. In this study, the concentration of hinokitiol in skin lotions was determined by HPLC with a visible light detector (450 nm) after pre-column derivatization with 4-(dimethylamino)azobenzene-4’-sulfonyl chloride (Dabsyl-Cl), a more economical reagent. A standard curve was obtained after derivatization with Dabsyl-Cl in borate buffer (pH 9.5) at 55°C for 10 min. The retention time of Dabsyl-hinokitiol was 6.8 min. The calibration plot was linear in the range of 1.25 to 40 μg/mL with a r2 value of 0.9991, and the lower limit of quantification and detection were 0.60 μg/mL (absolute amount of 0.86 ng/20μL injection, signal-to-noise ratio of 10:1) and 0.18 μg/mL (absolute amount of 0.26 ng/20μL injection, signal-to-noise ratio of 3:1), respectively. The coefficient of variation was less than 8.8%. Seven Dabsyl-paraben derivatives showed little interference with the peak of Dabsyl-hinokitiol. The developed system was used to determine the content of hinokitiol in two skin lotions. Addition-recovery tests gave satisfactory results.
机译：Hinokitiolis经常添加到个人护理产品中作为抗菌剂。我们以前建立了HPLC-UV方法，用于用4氟-7-硝基-2,1,3-苯并唑二唑柱衍生后测定皮肤乳液中的Hinokitiol。然而，标记试剂是昂贵的，并且羟基苯甲酸酯的降解产物的衍生物可以加入皮肤乳液作为一般防腐剂，干扰了羟基醇衍生物的峰。在这项研究中，通过HPLC与可见光检测器（450nm）与4-（二甲基氨基）偶氮苯-4'-磺酰氯（Dabsyl-Cl）的柱衍生化后，通过HPLC与可见光探测器（450nm）测定皮肤乳液中的浓度。经济试剂。在55℃下用硼酸盐缓冲液（pH9.5）与Dabsyl-Cl衍生化后获得标准曲线10分钟。二甲基 - 辛钛的保留时间为6.8分钟。校准图在1.25至40μg/ ml的范围内，R2值为0.9991，定量和检测的下限为0.60μg/ mL（绝对量为0.86ng /20μl注入，发出信号 - 噪声分别为10：1）和0.18μg/ ml（绝对量为0.26ng /20μl注射，3：1的信噪比）。变异系数小于8.8％。七种二甲基 - 羟基甲衍生物表现出对多甲基 - 辛酸峰的干扰很小。发达的系统用于确定两种皮肤乳液中的初生醇的含量。添加恢复测试得到了令人满意的结果。

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