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首页> 外文期刊>American Journal of Analytical Chemistry >Preparation of New Uric Acid Sensors Based on Iodide Selective Electrode
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Preparation of New Uric Acid Sensors Based on Iodide Selective Electrode

机译：基于碘化物选择性电极的新尿酸传感器的制备

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An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H_(2)O_(2) formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10~(-5) to 2 × 10~(-4) M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.
机译：通过使用具有尿液酶的碘化物选择性电极制备用于尿酸的电极。根据我们以前的研究，由10％TDMAI和PVC制备使用的碘化物选择性电极。通过在室温下将其在pH7磷酸盐缓冲液中保持20分钟，将酶固定在碘化物电极上。由尿酸反应形成的H_（2）O_（2）从反应细胞中存在的碘化物浓度的降低确定。潜在的变化在2×10〜（-5）至2×10〜（-4）m uric酸浓度（3-44mg尿酸/ 100ml血液）范围内。用新电极测定一些血液样品的尿酸含量，用比色法获得一致性。研究了pH，碘化物浓度，固定的酶量和操作温度的影响。没有观察到抗坏血酸，葡萄糖和尿素的干扰。

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