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首页> 外文期刊>Animals >Survey of Serum Amyloid A and Bacterial and Viral Frequency Using qPCR Levels in Recently Captured Feral Donkeys from Death Valley National Park (California)
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Survey of Serum Amyloid A and Bacterial and Viral Frequency Using qPCR Levels in Recently Captured Feral Donkeys from Death Valley National Park (California)

机译：血清淀粉样蛋白A和细菌和病毒频率使用QPCR水平从最近捕获的野生驴从死亡谷国家公园（加利福尼亚州）

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Feral donkey removal from state land has raised concerns in terms of disease transmission between equine species. Disease outbreaks may occur as a result of the relocation of animals to new environments. Virus and bacteria DNA load and serum amyloid A derived from the pathogenic processes that they involve were measured in recently captured donkeys. Blood and nasal swabs were collected from 85 donkeys (Death Valley National Park, Shoshone, California); 24 were retested after 30/60 days in the Scenic (Arizona) long-term holding facility co-mingled with feral donkeys from Arizona and Utah. Quantitative Real-Time PCR (qPCR) was performed to detect viral and bacterial genomic material (equine influenza A [EIV], equine rhinitis A and B viruses, AHV-2, AHV-3, AHV-5 and EHV-1, EHV-4, Streptococcus equi subspecies equi and zooepidemicus ,). Significant relations between behavior, body condition score, nasal discharge, and coughing were found in donkeys for which AHV-2 and Streptococcus zooepidemicus DNA was detected. Higher SAA concentrations were found in foals. AHV-2 and Streptococcus zooepidemicus DNA concentrations significantly differed between sampling moments ( p 0.05). In conclusion, donkeys do not appear to be a substantial risk for disease transmission to horses but could be if they carried strangles or other processes in which AHV-2 and Streptococcus zooepidemicus were involved.
机译：野生驴从州土地上移除已经提出了马匹疾病传播的担忧。由于动物迁移到新环境，可能会发生疾病爆发。病毒和细菌DNA负载和血清淀粉样蛋白衍生自涉及它们在最近捕获的驴中的致病过程。从85个驴（死亡谷国家公园，加利福尼亚州）收集血液和鼻拭子;在景区（亚利桑那州）长期控股设施中30/60天后重新测试了与亚利桑那州和犹他州的野生驴合并。进行定量实时PCR（QPCR）以检测病毒和细菌基因组材料（马流感A A和B病毒，AHV-2，AHV-3，AHV-5和EHV-1，EHV- 4，链球菌等亚种Equi和Zooepidemicus，）。在驴中发现行为，身体状况得分，鼻排放和咳嗽之间的显着关系，用于检测到AHV-2和链球菌Zoopidemicus DNA。在马驹中发现了更高的Saa浓度。 AHV-2和链球菌ZOOOPIDEMEDUS DNA浓度在取样时刻显着不同（P <0.05）。总之，驴似乎并不是疾病传播对马匹的大量风险，但如果他们携带涉及AHV-2和链球菌毒病患病的扼杀或其他过程。

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