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首页> 外文期刊>Aquaculture Reports >Characterization of pathological changes and immune-related gene expression in yellow drum (Nibea albiflora) in response to Pseudomonas plecoglossicida and poly I:C challenge
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Characterization of pathological changes and immune-related gene expression in yellow drum (Nibea albiflora) in response to Pseudomonas plecoglossicida and poly I:C challenge

机译：黄牛（Nibea Albiflora）对黄牛（Nibea Albiflora）的病理变化和免疫相关基因表征的表征，响应于假单胞菌（Nibea Albiflora）和Poly I：C攻击

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The yellow drum ( Nibea albiflora ; Na ) constitutes an important seafood fish species that is subjected to intensive cage culture in China. The fish is susceptible to infection by bacteria and virtual pathogens, which results in considerable economic loss. In the present study, the pathogenic characteristics and immune response of the yellow drum were analyzed after Pseudomonas plecoglossicida or poly I:C challenge. Tissue sections revealed cell vacuolization in the liver and obvious macrophage infiltration in the spleen following P. plecoglossicida infection, whereas these tissues exhibited no significant lesions upon poly I:C challenge. Superoxide dismutases (SODs) and heat shock proteins (HSPs), which play important roles in the immune response of fish in defense against environmental threats or stresses, were further analyzed. The quantitative real time PCR (qPCR) analysis showed that mRNAs for intracellular copper/zinc-SOD ( Na SOD1), manganese SOD ( Na SOD2), extracellular copper/zinc-SOD ( Na SOD3), HSP70, and HSP90 were expressed in 11 tested tissues including high levels in the liver, spleen, kidney, and gill; further significant increase was observed in the liver, spleen, and gill following P. plecoglossicida or poly I:C challenge. Moreover, the activities of total SOD and the contents of NaHSP70 and NaHSP90 in the liver were up-regulated after P. plecoglossicida or poly I:C challenge, which was generally similar to the fluctuations of mRNA expression in the tissue. The results showed that the dynamic changes in SODs and HSPs exhibited a similar tendency, which might indicate a coordinate action against P. plecoglossicida or poly I:C challenge. These findings will contribute towards better understanding of the biological process involved in defending against pathogenic bacteria and virtual challenge, as well as provide insight regarding the molecular mechanisms of the initial immune response in the yellow drum.
机译：黄色鼓（Nibea Albiflora; Na）构成了中国密集的笼养文化的重要海鲜鱼种。鱼类易受细菌和虚拟病原体感染的影响，这导致了相当大的经济损失。在本研究中，在假鼠Plecoglossicida或Poly I：C攻击后分析了黄色鼓的致病特性和免疫应答。组织切片揭示了肝脏中的细胞真空，在P.Plecoglossicida感染后脾脏中的明显巨噬细胞浸润，而这些组织在Poly I：C攻击时表现出显着的病变。进一步分析了超氧化物歧化酶（SODS）和热休克蛋白（HSP），其在防御环境威胁或压力的鱼类免疫应答中起重要作用。定量实时PCR（QPCR）分析表明，11例表达了11点11例对细胞内铜/锌-SOD（NA SOD1），锰SOD（NA SOD2），细胞外铜/锌-SOD（Na SOD3），HSP70和HSP90的MRNA测试组织，包括肝脏，脾脏，肾脏和鳃中的高水平;在P.Plecoglossicida或Poly I：C攻击之后，在肝脏，脾脏和鳃中观察到进一步显着增加。此外，在P.Plecoglossicida或Poly I：C攻击后，肝脏中NaHSP70和NaHSP90的总SOD和NaHSP90的含量和NaHSP90的活性均上调，这通常与组织中mRNA表达的波动相似。结果表明，SODS和HSP的动态变化表现出类似的趋势，这可能表明对P.Plecoglossicida或Poly I：C攻击的坐标作用。这些调查结果将有助于更好地理解参与致病性细菌和虚拟挑战措施的生物学进程，并提供关于黄色鼓中初始免疫应答的分子机制的洞察力。

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