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首页> 外文期刊>Arquivo Brasileiro de Medicina Veterinaria e Zootecnia >Análise histológica de cartilagens elásticas tratadas com solu??o alcalina
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Análise histológica de cartilagens elásticas tratadas com solu??o alcalina

机译：用碱性溶液处理的弹性软骨组织学分析

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The elastic cartilage is composed by chondroblasts and chondrocytes, extracellular matrix and surrounded by perichondrium. It has a low regeneration capacity and is a challenge in surgical repair. One of obstacles in engineering a structurally sound and long-lasting tissue is selecting the most appropriate scaffold material. One of the techniques for obtaining biomaterials from animal tissues is the decellularization that decreases antigenicity. In this work, alkaline solution was used in bovine ear elastic cartilages to evaluate the decellularization and the architecture of the extracellular matrix. The cartilages were treated in alkaline solution (pH13) for 72 hours and lyophilized to be compared with untreated cartilages by histological analysis (hematoxylin-eosin, Masson's trichrome and Verhoeff slides). Areas of interest for cell counting and elastic fiber quantification were delineated, and the distribution of collagen and elastic fibers and the presence of non-fibrous proteins were observed. The results demonstrated that the alkaline solution caused 90% decellularization in the middle and 13% in the peripheral region, and maintenance of the histological characteristics of the collagen and elastic fibers and non-fibrous protein removal. It was concluded that the alkaline solution was efficient in the decellularization and removal of non-fibrous proteins from the elastic cartilages of the bovine ear.
机译：弹性软骨由软骨细胞和软骨细胞组成，细胞外基质和脑鞘包围。它具有较低的再生能力，是手术修复的挑战。工程在结构上的障碍物之一结构声音和持久的组织是选择最合适的支架材料。用于从动物组织获得生物材料的技术之一是降低抗原性的脱细胞化。在这项工作中，碱性溶液用于牛耳弹性软骨，以评估细胞外基质的脱细胞化和结构。用碱性溶液（pH13）处理包裹物72小时，并通过组织学分析（血毒素 - 曙红，Masson的richrome和Verhoeff载玻片）与未经处理的软骨进行冻干。对细胞计数和弹性纤维量化的感兴趣区域描绘，并且观察到胶原蛋白和弹性纤维的分布和非纤维蛋白的存在。结果表明，碱性溶液在中间区域中引起90％的脱细胞化和13％，以及维持胶原蛋白和弹性纤维的组织学特征和非纤维蛋白质去除。得出结论是，碱性溶液在牛耳的弹性软骨中的脱细胞化和除去非纤维蛋白质有效。

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