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首页> 外文期刊>Arquivo Brasileiro de Medicina Veterinaria e Zootecnia >Eficácia da associa??o TES-TRIS ou TRIS com lipoproteína de baixa densidade sobre a longevidade in vitro de sêmen refrigerado de búfalos
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Eficácia da associa??o TES-TRIS ou TRIS com lipoproteína de baixa densidade sobre a longevidade in vitro de sêmen refrigerado de búfalos

机译：TES-TRIS或TRIS关联与低密度脂蛋白对缓冲器冷藏精液体外长寿的有效性

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This study investigated in vitro the efficacy of four different extenders (TES-TRIS and TRIS with LDL low-density lipoprotein at concentrations of 10 or 5%) on the longevity of buffalo sperm in the refrigeration process at 5oC. Sperm motility was assessed every 24 hours up to 72 hours of incubation using computer assisted sperm analysis and sperm membrane integrity was examined by the hypoosmotic test (HOST) at T1, T24, T48 and T72 hours. Eleven buffaloes (1 ejaculate per buffalo) of the Murrah breed were used, ranging in age from 4 to 5 years. Immediately after collection, each ejaculate was fractionated into 4 aliquots, and each aliquot was diluted in one of four diluents to obtain 50x106SPTZ/mL. The samples were packed in 0.5mL straws and refrigerated (-0.25°C/min) to 5°C and maintained at this temperature until evaluation. Prior to evaluation the samples were heated at 37°C for 30 seconds. The statistical package used for analysis was STATA 12.0 "Statistical Analysis Software" and means were compared by the Friedman test (P0.05). The results of sperm kinetics and HOST indicate that the TRIS diluent with 10% LDL could be a promising alternative for semen refrigeration at 5oC, to be used in conventional and fixed time artificial insemination.
机译：该研究在体外研究了四种不同的增量剂（TES-TRIS和TRIS在LDL低密度脂蛋白的浓度为10或5％）上的疗效对5℃的冷冻过程中的水牛精子的寿命。使用计算机辅助精子分析每24小时进行每24小时孵育每24小时的精子运动，并且通过T1，T24，T48和T72小时的低管测试（宿主）检查精子膜完整性。使用Murrah品种的11次水牛（每次射精），从4至5年的年龄范围内。在收集后，将每次射精分馏成4个等分试样，并在四种稀释剂中的一种中稀释每种等分试样，得到50×10 6 SPTZ / mL。将样品用0.5mL吸管包装在0.5mL秸秆中，冷藏（-0.25℃/ min）至5℃并保持在该温度直至评价。在评价之前，将样品在37℃下加热30秒。用于分析的统计包是STATA 12.0“统计分析软件”，并通过FREDEMAN TEST进行比较（P <0.05）。精子动力学和宿主的结果表明，具有10％LDL的Tris稀释剂可能是5℃的精液制冷的有希望的替代方案，以用于常规和固定时间的人工授精。

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