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首页> 外文期刊>Arquivo Brasileiro de Medicina Veterinaria e Zootecnia >Saccharomyces cerevisiae como agente probiótico e possível adsorvente de aflatoxina B1 em condi??es simuladas do trato intestinal de peixes
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Saccharomyces cerevisiae como agente probiótico e possível adsorvente de aflatoxina B1 em condi??es simuladas do trato intestinal de peixes

机译：Saccharomyces Cerevisiae作为益生菌和可能的鱼类肠道模拟条件下的黄曲霉毒素B1的吸附剂

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摘要

The aim of this study was to evaluate in vitro the probiotic potential and absorption of Saccharomyces cerevisiae for the aflatoxin B1 in simulated fish intestinal tract conditions. Three yeast strains were used, two from brewery: S. cerevisiae RC1 and S. cerevisiae RC3 and one from a fish farming environment: S. cerevisiae A8L2. The selected yeasts were subjected to the following in vitro tests: homologous inhibition, self-aggregation, co-aggregation, antibacterial activity, gastrointestinal conditions tolerance and adsorption of AFB1. All S. cerevisiae strains showed good capability of self-aggregation and co-aggregation with pathogenic bacteria. All yeast strains were able to survive the gastrointestinal conditions. In acidic conditions, the factors (strain vs. time) had interaction (P=0.0317), resulting in significant variation among the strains tested in the time periods analyzed. It was observed that there was also interaction (P=0.0062) in intestinal conditions, with an increased number of cells in the 12-hour period for all strains tested. In the adsorption test, the A8L2 strain was statistically more effective (P0.005) for both AFB1 concentrations evaluated in this study (10 and 25ng/mL). Thus, it was observed that the strains of S. cerevisiae have potential probiotic and adsorbent of AFB1.
机译：本研究的目的是评估在模拟鱼类肠道条件下对黄曲霉毒素B1的益生菌潜力和吸收酿酒酵母的益生菌潜力和吸收。使用了三种酵母菌株，两种来自啤酒厂：S.Cerevisiae RC1和S.Cerevisiae RC3和来自鱼类养殖环境的培训，酿酒酵母环境：S.Cerevisiae A8L2。将所选择的酵母进行以下体外试验：同源抑制，自聚集，共聚，抗菌活性，胃肠环境耐受性和AFB1的吸附。所有S.酿酒酵母菌株都表现出良好的自我聚集和与致病细菌共聚的能力。所有酵母菌株都能够存活胃肠道条件。在酸性条件下，因子（菌株对时间）具有相互作用（p = 0.0317），导致分析时间段测试的菌株中的显着变化。观察到，在肠道条件下也存在相互作用（p = 0.0062），在测试所有菌株的12小时内具有增加的细胞数量增加。在吸附试验中，对于本研究（10和25ng / ml）评估的两种AFB1浓度，A8L2菌株对AFB1浓度的统计学上更有效（P <0.005）。因此，观察到S.Cerevisiae的菌株具有潜在的益生菌和AFB1的吸附剂。

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