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首页> 外文期刊>International Journal of Electrochemical Science >A Selective Medium for Pyocyanin-dependent Fast Electrochemical Detection of Pseudomonas aeruginosa in Environmental Microbial Samples
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A Selective Medium for Pyocyanin-dependent Fast Electrochemical Detection of Pseudomonas aeruginosa in Environmental Microbial Samples

机译：依赖于环境微生物样品中铜绿假单胞菌的粘稠快速电化学检测的选择性培养基

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In this work, a selective medium was developed for pyocyanina-dependent qualitative detection of Pseudomonas aeruginosa in raw microbial samples using electrochemical analysis. To obtain an optimum nutritive formulation, the effect of different media (BYEB, LB, SDB, MHB, TSB, and NB), positive ions (K+, Na+, Ca2+, Fe3+, and Mg2+) and selected additives (1-naphthylamine, acetamide, and benzylpenicillin sodium) on the amount of synthesized pyocyanin were investigated and compared. NB was found to be the most favourable medium for the electrochemical detection of pyocyanin, and Mg2+ was shown to be the most effective supporting additive ion for the detection. 1-Naphthylamine was shown to be the most effective supporting antibiotic reagent when supplied in the medium to enhancing the presence of pyocyanina compared to the other two antibiotics. The results indicated that the improved selective medium (ISM) can effectively inhibit/limit the growth of various microorganisms other than P. aeruginosa and obviously enhance the production of pyocyanin in environmental samples that contained P. aeruginosa. This study provided a method for simple and fast electrochemical detection of P. aeruginosa in complex microbial samples.
机译：在这项工作中，使用电化学分析，在原始的微生物样品中依赖于Pseudomonas铜绿假单胞菌的Psocyanina依赖性检测选择性培养基。为了获得最佳的营养制剂，不同培养基（BYEB，LB，SDB，MHB，TSB和NB）的作用，正离子（K +，Na +，Ca2 +，Fe3 +和Mg2 +）和选定的添加剂（1-萘胺，乙酰胺和苄基培素钠）研究并比较了合成的碧糊酶量。发现Nb是苯硅蛋白的电化学检测最有利的介质，并且显示Mg2 +是检测的最有效的支持性添加离子。当在培养基中供应时，1-萘胺被显示为最有效的抗生素试剂，以增强与其他两种抗生素相比氏菌的存在。结果表明，改进的选择性培养基（ISM）可以有效地抑制/限制P.铜绿假单胞菌以外的各种微生物的生长，并明显增强含有P.铜绿假单胞菌的环境样品中碧粘蛋白的生产。本研究提供了一种用于复杂微生物样品中的P.铜绿假单胞菌的简单和快速电化学检测方法。

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