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Development of Microsatellite Markers for the Hermatypic Coral Porites lutea

机译:对珊瑚诗百叶菌Lutea的微卫星标记的研制

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By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed 20 microsatellite markers for the stony coral Porites lutea , meanwhile, validated and excluded 43 microsatellite markers of its symbiotic zooxanthella. These markers of P. lutea were genotyped in one wild population (n=16) from the southwest of Hainan Island fringing reefs. Nineteen of the 20 markers showed polymorphism. The number of alleles was 2~7 (mean 3.5±1.8), and the observed heterozygosity ( H o ) and expected heterozygosity ( H e ) were 0.06~0.94 (mean 0.34±0.29) and 0.18~0.83 (mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium (HWE) after multiple comparison correction for Bonferroin ( P HWE 0.05). This is the first set of microsatellite markers of P. lutea . They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.
机译:通过挖掘Genbank中Porites物种的EST和基因组微卫星序列资源,并使用间隙扩增和重构,我们为石珊瑚Porites Lutea开发了20个微卫星标记,同时,验证和排除了其共生Zooxanthishers的43个微卫星标志物。 P. Lutea的这些标志物在海南岛雷丁礁西南部的一个野生群体(n = 16)中进行了基因分型。 20个标记的十九个标记显示多态性。等位基因数为2〜7(平均3.5±1.8),观察到的杂合子(H O)和预期的杂合性(H E)为0.06〜0.94(平均0.34±0.29)和0.18〜0.83(平均0.57±0.20) , 分别。在对Bonferroin的多种比较校正后,四个标记仍然偏离了Hardy-Weinberg平衡(HWE)(P HWE <0.05)。这是第一组P. Lutea的微卫星标记。它们应该对该物种的遗传多样性和连通性以及濒临灭绝的珊瑚礁生态系统和珊瑚礁建设珊瑚群体的研究有用。

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