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首页> 外文期刊>International Journal of Microbiology >Genomic and Pathogenic Characteristics of Virulent Newcastle Disease Virus Isolated from Chicken in Live Bird Markets and Backyard Flocks in Kenya
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Genomic and Pathogenic Characteristics of Virulent Newcastle Disease Virus Isolated from Chicken in Live Bird Markets and Backyard Flocks in Kenya

机译：在肯尼亚的现场鸟类和后院鸡群中鸡的毒性新城疾病病毒基因组和致病特征

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Newcastle disease (ND) causes significant economic losses in the poultry industry in developing countries. In Kenya, despite rampant annual ND outbreaks, implementation of control strategies is hampered by a lack of adequate knowledge on the circulating and outbreak causing-NDV strains. This study reports the first complete genome sequences of NDV from backyard chicken in Kenya. The results showed that all three isolates are virulent, as assessed by the mean death time (MDT) and intracerebral pathogenicity index (ICPI) in specific antibody negative (SAN) embryonated eggs and 10-day-old chickens, respectively. Also, the polybasic amino acid sequence at the fusion-protein cleavage site had the motif 112RRQKRFV118. Histopathological findings in four-week-old SPF chicken challenged with the NDV isolates KE001, KE0811, and KE0698 showed multiple organ involvement at five days after infection with severe effects seen in lymphoid tissues and blood vessels. Analysis of genome sequences obtained from the three isolates showed that they were 15192 base pair (bp) in length and had genomic features consistent with other NDV strains, the functional sites within the coding sequence being highly conserved in the sequence of the three isolates. Amino acid residues and substitutions in the structural proteins of the three isolates were similar to the newly isolated Tanzanian NDV strain (Mbeya/MT15). A similarity matrix showed a high similarity of the isolates to NDV strains of class II genotype V (89–90%) and subgenotype Vd (95–97%). Phylogenetic analysis confirmed that the three isolates are closely related to NDV genotype V strains but form a distinct cluster together with NDV strains from the East African countries of Uganda and Tanzania to form the newly characterized subgenotype Vd. Our study provides the first description of the genomic and pathological characteristics of NDV of subgenotype Vd and lays a baseline in understanding the evolutionary dynamics of NDV and, in particular, Genotype V. This information will be useful in the development of specific markers for detection of viruses of genotype V and generation of genotype matched vaccines.
机译：新城疫（ND）在发展中国家造成家禽业的重大经济损失。在肯尼亚，尽管年度ND爆发猖獗的爆发，但对控制策略的实施受到对循环和爆发引起的血管NDV菌株的充分知识受到阻碍。本研究报告了在肯尼亚的后院鸡的第一个完整的NDV完整基因组序列。结果表明，所有三个分离物都是毒性，如分别的平均死亡时间（MDT）和脑内致病性指数（ICPI）分别在特异性抗体（SAN）胚胎卵和10日龄鸡中的脑内致病性指数（ICPI）评估。而且，融合蛋白裂解位点的多元氨基酸序列具有MOTIF 112RRQKRFV118。用NDV分离物Ke001，Ke0811和Ke0698挑战的四周历史SPF鸡肉中的组织病理学发现显示，在淋巴组织和血管中患有严重影响后的5天显示多器官受累。从三个分离株获得的基因组序列的分析表明它们的长度为15192个碱基对（BP），并且具有与其他NDV菌株一致的基因组特征，编码序列内的功能位点在三个分离株的序列中高度保守。三个分离株结构蛋白中的氨基酸残基和取代与新分离的坦桑尼亚NDV菌株（MBEYA / MT15）类似。相似性基质显示出II类基因型V（89-90％）和亚型型VD（95-97％）的NDV菌株的高相似性。系统发育分析证实，三种分离株与NDV基因型V株密切相关，但形成了不同的群体与来自乌干达和坦桑尼亚的东非国家的NDV菌株组成，形成新特征的亚因素vd。我们的研究提供了亚因素VD的NDV基因组和病理特征的第一个描述，并在理解NDV的进化动态方面奠定基线，特别是基因型V.该信息对于检测特定标记的发展将是有用的基因型V的病毒V和基因型匹配疫苗的产生。

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