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首页> 外文期刊>International Journal of Photoenergy >Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells
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Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells

机译：KATP 2-转染的HEK-293细胞KATP通道的光致调节

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Background and Objective. ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability. To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel.Study Design/Materials and Methods. Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation. A laser beam of Ga-As 808 nm at 5 mW/cm2was used in experiments. A one-way ANOVA test followed by apost hocStudent-Newman-Keuls test was used to assess the statistical differences between data groups.Results. Obvious openings of KATP channels of Kir6.2-transfected HEK-293 cells and excised patches were recorded during and after low intensity 808 nm laser irradiation. Compared with the channels that did not undergo irradiation, open probability, current amplitude, and dwell time of KATP channels after irradiation improved.Conclusions. Low intensity 808 nm laser irradiation may activate membrane KATP channels of Kir6.2-transfected HEK-293 cells and in excised patches.
机译：背景和目标。 ATP敏感钾（KATP）通道将细胞代谢与兴奋性相结合。为了探讨KATP通道在蜂窝光生物调节中的作用，我们设计了实验，研究了低强度808nm激光辐射对膜KATP通道活性的实验。研究/材料和方法。编码Kir6.2的质粒在培养的哺乳动物HEK-293细胞中构建和异源地表达。 PATCH-CLAMP和数据采集系统用于在照射之前和之后记录KATP通道电流。在实验中使用的5mW / cm2was的Ga-As 808nm的激光束。单向ANOVA测试，然后是Apost Hocstudent-Newman-Keuls测试用于评估数据组之间的统计差异。结果。在低强度808nm激光照射期间和之后记录了KiR6.2转染的HEK-293细胞的明显开口KATP通道和低强度808nm激光辐照。与不经历辐射，打开概率，电流幅度和katp通道的停留时间的通道相比，辐照改善后。结论。结论。低强度808nm激光辐射可以激活Kir6.2转染的HEK-293细胞的膜KATP通道和切除的斑块。

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