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Experimental study for evaluation of a low-cost spray cryotherapy system

机译:低成本喷雾冷冻疗法评价的实验研究

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Objective To evaluate the feasibility and applicability of a low-cost cryotherapy system. Methods Experimental study with 25kg Landrace pigs submitted to a longitudinal cervico-thoraco-abdominal incision for exposure of the trachea, thorax and abdomen. The tissues were frozen by continuous spray application at different periods of time (5, 10 and 15 seconds). Spray cryotherapy was performed using a fluorinated gas (tetrafluorethane) delivered at - 47°C temperature (DermaFreeze ? , Emdutos; ANVISA registration 80409950001; price R$ 394,00). via an adapted, disposable 1.8mm cholangiography catheter (Olympus; price R$ 280,00). The specimens were resected for histopathological analysis. Results Thirty samples were obtained from ten different organs and divided according to spray cryotherapy application time. System activation for 5, 10 or 15 seconds led to consumption of 14g, 27g and 40g of gas respectively (average gas consumption, 2.7g/s using a 1.8mm catheter). The system comprising a spray tube and catheter proved user-friendly and effective, with constant gas dispersion and adequate tissue freezing. In spite of effective freezing, microscopy failed to reveal tissue changes. This may have reflected methodological constraints precluding evaluation at tissue damage peak time (48 hours). Conclusion The low-cost spray cryotherapy system proved feasible and safe.
机译:目的探讨低成本冷冻疗法系统的可行性和适用性。方法采用25kg Landrace猪的实验研究,提交给纵向宫颈切口暴露的气管,胸部和腹部。在不同的时间(5,10和15秒)下通过连续喷涂施用冷冻组织。使用氟化气体(四氟乙烷)在47℃温度下进行喷雾冷冻疗法(Dermafreeze?,Emdutos; Anvisa注册80409950001;价格R $ 394,00)。通过适应的一次性1.8mm胆管造影导管(奥林巴斯;价格r $ 280,00)。切除标本以进行组织病理学分析。结果从十种不同的器官获得30个样品,并根据喷雾冷冻疗法施用时间分开。系统激活5,10或15秒导致分别为14g,27g和40g气体(平均气体消耗,2.7g / s使用1.8mm导管)。该系统包括喷射管和导管被证明是用户友好且有效的,具有恒定的气体分散和足够的组织冻结。尽管有效冻结,显微镜未能露出组织变化。这可能对组织损伤峰值时间(48小时)的评估反映了方法。结论低成本喷雾冷冻疗法系统证明可行安全。

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