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首页> 外文期刊>Environmental and Climate Technologies >Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria
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Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria

机译：CopTotermes Coptotmes Courtomathus肠道生产的木质素溶解

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Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis.
机译：木质纤维素生物质的最大利用在降解克钙素木质素聚合物时是目的的。常规方法采用的脱烃方法需要高能量和化学品输入，导致高毒性污水的释放。本研究研究了CopTotermes CopTotermes CopTotermes Cutgignathus的能力。将木质素溶解的酶的生产在具有牛皮油木蛋白作为唯一碳源的充气浸没式发酵体系中进行。降解实验在30℃下进行7天，pH7。三个潜在的木质素降解剂，其被鉴定为芽孢杆菌SP。，Lysinibacillus Sp。和acinetobacter sp。成功孤立。细胞外木质素溶解酶的细菌生长和分泌证实了鉴定的菌株的牛皮油的代谢。 Lignin降解剂的新型Lignin降解剂分别显示出最高锰过氧化物酶（76.36±15.74 U / L）和漆酶活性（76.36±16.82 U / L），分别在孵育7和6天后，而木质素过氧化物酶的最大活性（262.49±0.92在芽孢杆菌培养上清液中7天后记录U / L）。关于分泌酶的活性，可以探讨这些细菌菌株的木质素降解潜力，可在工业过程中木质纤维素生物量的甘油化中探索，如制浆，生物乙醇生产，精细化学品和材料合成。

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