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首页> 外文期刊>Experimental Animals >Lumbar Intervertebral Disc Puncture under C-arm Fluoroscopy: a New Rat Model of Lumbar Intervertebral Disc Degeneration
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Lumbar Intervertebral Disc Puncture under C-arm Fluoroscopy: a New Rat Model of Lumbar Intervertebral Disc Degeneration

机译：C形臂透视下的腰椎椎间盘刺穿：腰椎椎间盘变性的新大鼠模型

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To establish a minimally invasive rat model of lumbar intervertebral disc degeneration (IDD) to better understand the pathophysiology of the human condition. The annulus fibrosus of lumbar level 4–5 (L4-5) and L5-6 discs were punctured by 27-gauge needles using the posterior approach under C-arm fluoroscopic guidance. Magnetic resonance imaging (MRI), histological examination by hematoxylin and eosin (H&E) staining, and reverse transcription polymerase chain reaction (RT-PCR) were performed at baseline and 2, 4, and 8 weeks after disc puncture surgery to determine the degree of degeneration. All sixty discs (thirty rats) were punctured successfully. Only two of thirty rats subjected to the procedure exhibited immediate neurological symptoms. The MRI results indicated a gradual increase in Pfirrmann grade from 4 to 8 weeks post-surgery ( P <0.05), and H&E staining demonstrated a parallel increase in histological grade ( P <0.05). Expression levels of aggrecan, type II collagen ( Col2 ), and Sox9 mRNAs, which encode disc components, decreased gradually post-surgery. In contrast, mRNA expression of type I collagen ( Col1 ), an indicator of fibrosis, increased ( P <0.05). The procedure of annular puncture using a 27-gauge needle under C-arm fluoroscopic guidance had a high success rate. Histological, MRI, and RT-PCR results revealed that the rat model of disc degeneration is a progressive pathological process that is similar to human IDD.
机译：建立腰椎椎间盘退化（IDD）的微创大鼠模型，以更好地了解人体状况的病理生理学。腰部4-5（L4-5）和L5-6圆盘的环形纤维蕈类在C形臂透视引导下使用后验方法穿刺27··尺寸针。磁共振成像（MRI），通过溶血素和曙红（H＆E）染色和逆转录聚合酶链反应（RT-PCR）的组织学检查在椎间盘刺穿手术后的2,4,4周，以确定退化。所有六十圆盘（30只大鼠）都成功刺破。患有该程序的三十只大鼠中只有两个表现出直接神经系统症状。 MRI结果表明，手术后4至8周的PFIRRMAN级别逐渐增加（P <0.05），H＆E染色表现出组织学等级的平行增加（P <0.05）。 Exclocan的表达水平，II型胶原（COL2），以及编码盘组分的SOX9 mRNA，逐渐术后降低。相反，I型胶原蛋白（COL1）的mRNA表达，纤维化指示剂增加（P <0.05）。在C形臂荧光引导下使用27型针的环形穿刺程序具有高成功率。组织学，MRI和RT-PCR结果表明，盘变性的大鼠模型是一种类似于人IDD的渐进病理由。

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