...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genome Integrity >Development of dose-response calibration curve for dicentric chromosome induced by X-rays
【24h】

Development of dose-response calibration curve for dicentric chromosome induced by X-rays

机译：X射线引起的DicEntric染色体剂量响应校准曲线的研制

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chromosome aberration is a biomarker that has been used as a standard tool in biological dosimetry (biodosimetry) of individuals after exposure to ionizing radiation. It is based mainly on the induction of dicentric chromosomes – one of the radiation-induced biological effects, in order to correlate them with radiation dose. In this study, a dose calibration curve for X-rays was generated by using the dicentric assay and by fitting the data to both Chromosomal Aberration Calculation Software and Dose Estimate programs to compare the output of each method. Peripheral blood samples from four nonsmoker healthy donors were irradiated with various doses ranging from 0 to 4 Gy with 250 kV or 122 keV X-rays at a dose rate of 0.17 Gy/min. The irradiated blood was cultured, harvested, and analyzed according to the standard procedure as described by the International Atomic Energy Agency with slight modifications. The dose-response calibration data for dicentrics were fitted with the linear-quadratic model (Ydic = 0.03987D2 + 0.00651D). The dose-response calibration curve obtained in this research was comparable to other estimations with similar radiation quality and dose rates. The results in this research convinced us in sustaining a biodosimetry using a dose-response calibration curve in our laboratory.
机译：染色体畸变是在暴露于电离辐射后被用作生物剂量（生物渗透压）中的标准工具的生物标志物。它主要是基于Dicentric染色体的诱导 - 辐射诱导的生物效应之一，以便将它们与辐射剂量相关联。在该研究中，通过使用Dicentric测定来产生X射线的剂量校准曲线，并通过将数据拟合到染色体像差计算软件和剂量估计程序来比较每种方法的输出。来自四个Nonsmoker健康供体的外周血样品用各种剂量的速度照射，其各种剂量为0至4Gy，剂量率为0.17 gy / min。速率为250kV或122keV X射线。根据国际原子能机构描述的标准程序，培养，收获并分析辐照血液，并进行培养，收获和分析。 DICENTICS的剂量响应校准数据配有线性二次模型（YDIC = 0.03987D2 + 0.00651D）。本研究中获得的剂量响应校准曲线与具有相似辐射质量和剂量率的其他估计相当。这项研究的结果确信我们在我们实验室中使用剂量反应校准曲线维持生物渗透液。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号