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RNA sequencing analysis of salt tolerance in soybean (Glycine max)

机译：大豆盐耐盐性RNA测序分析（甘氨酸）

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Salt stress causes foliar chlorosis and scorch, plant stunting, and eventually yield reduction in soybean. There are differential responses, namely tolerance (excluder) and intolerance (includer), among soybean germplasm. However, the genetic and physiological mechanisms for salt tolerance is complex and not clear yet. Based on the results from the screening of the RA-452 x Osage mapping population, two F4:6 lines with extreme responses, most tolerant and most sensitive, were selected for a time-course gene expression study in which the 250?mM NaCl treatment was initially imposed at the V1 stage and continued for 24?h (hrs). Total RNA was isolated from the leaves harvested at 0, 6, 12, 24?h after the initiation of salt treatment, respectively. The RNA-Seq analysis was conducted to compare the salt tolerant genotype with salt sensitive genotype at each time point using RNA-Seq pipeline method. A total of 2374, 998, 1746, and 630 differentially expressed genes (DEGs) between salt-tolerant line and salt-sensitive line, were found at 0, 6, 12, and 24?h, respectively. The expression patterns of 154 common DEGs among all the time points were investigated, of which, six common DEGs were upregulated and seven common DEGs were downregulated in salt-tolerant line. Moreover, 13 common DEGs were dramatically expressed at all the time points. Based on Log2 (fold change) of expression level of salt-tolerant line to salt-sensitive line and gene annotation, Glyma.02G228100, Glyma.03G226000, Glyma.03G031000, Glyma.03G031400, Glyma.04G180300, Glyma.04G180400, Glyma.05?g204600, Glyma.08G189600, Glyma.13G042200, and Glyma.17G173200, were considered to be the key potential genes involving in the salt-tolerance mechanism in the soybean salt-tolerant line.
机译：盐胁迫导致叶面萎缩和烧焦，植物衰退，最终屈服于大豆的产量。在大豆种质中，存在差异响应，即耐受性（不包括）和不容忍（Includer）。然而，耐盐性的遗传和生理机制是复杂的，尚未清晰。基于筛选RA-452 X OSACE测绘群的结果，选择两个F4：6线具有极端响应，最耐受性和最敏感的，用于时间课程基因表达研究，其中250毫米NACL治疗最初施加在V1阶段，并持续24？H（HRS）。分别在盐处理开始后从0,6,12,24μH收获的叶片中分离总RNA。进行RNA-SEQ分析以将耐盐基因型与使用RNA-SEQ管道方法的每个时间点进行比较。在0,6,12和24℃下，共有2374,998,1746和630型差异表达基因（可在盐敏线之间进行差异表达基因（DEG）。研究了154个常见的常见次数中的表达模式，其中，上调了六种常见的次数，并在耐盐管线中下调7种常见的次数。此外，在所有时间点都显着表达了13个常见的常见次数。基于LOG2（折叠变化）的盐耐盐线和基因盐敏线和基因注释，Glyma.03g226000，Glyma.03g031000，Glyma.03g031400，Glyma.04g180300，Glyma.04g180400，Glyma.04g180400 05？G204600，Glyma.08G189600，Glyma.13G042200和Glyma.17G173200被认为是涉及大豆耐盐线中的耐盐机制的关键潜在基因。

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