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首页> 外文期刊>Genomics >Transcriptome sequencing of active buds from Populus deltoides CL. and Populus?×?zhaiguanheibaiyang reveals phytohormones involved in branching
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Transcriptome sequencing of active buds from Populus deltoides CL. and Populus?×?zhaiguanheibaiyang reveals phytohormones involved in branching

机译：来自杨树熟菌Cl的活性芽的转录组测序。和杨树？×？Zhaiguanheibaiyang揭示了涉及分支的植物激素

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Branching in woody plants affects their ecological benefits and impacts wood formation. To obtain genome-wide insights into the transcriptome changes and regulatory mechanisms associated with branching, we performed high-throughput RNA sequencing to characterize cDNA libraries generated from active buds of Populus deltoides CL. ‘zhonglin2025’ (BC) and Populus?×?zhaiguanheibaiyang (NC). NC has more branches than BC and rapid growth. We obtained a total of 198.2 million high-quality clean reads from the NC and BC libraries. We detected 3543 differentially expressed genes (DEGs) between the NC and BC libraries; 1418 were down-regulated and 2125 were up-regulated. Gene ontology functional classification of the DEGs indicated that they included 89 genes that encoded proteins related to hormone biosynthesis, 364 genes related to hormone signaling transduction, and 104 related to the auxin efflux transmembrane transporter. We validated the expression profiles of 16° by real-time quantitative PCR and found that their expression patterns were similar to those obtained from the high-throughput RNA sequencing data. We also measured the hormone content in young buds of BC and NC by high-pressure liquid chromatography. In this study, we identified global hormone regulatory patterns and differences in gene expression between NC and BC, and constructed a hormone regulatory network to explain branching in Populus buds. In addition, candidate genes that may be useful for molecular breeding of particular plant types were identified. Our results will provide a starting point for future investigations into the molecular mechanisms of branching in Populus.
机译：木质植物中的分支会影响其生态效益，并影响木材形成。为了获得进入与分支相关的转录组变化和调节机制的基因组洞察，我们进行了高通量RNA测序，以表征从Populus Deltoides Cl的活性芽产生的cDNA文库。 '中林2025'（BC）和杨树？×？Zhaiguanheibaiyang（NC）。 NC具有比BC和快速增长更多的分支。我们从NC和BC库中获得了19820万的高质量清洁读数。我们在NC和BC文库之间检测到3543次差异表达的基因（DEGS）; 1418被调节，2125次上调。基因本体的功能分类表明它们包括89个基因，其编码与激素生物合成相关的蛋白质，与激素信号转导有关的364个基因，以及与促进型流动式跨膜转运蛋白转运蛋白有关的104个。我们通过实时定量PCR验证了16°的表达谱，发现它们的表达模式类似于从高通量RNA测序数据获得的那些。我们还通过高压液相色谱法测量了BC和NC的幼芽中的激素含量。在这项研究中，我们确定了Nc和Bc之间的全球激素调节模式和基因表达的差异，并构建了一种激素调节网络，用于解释杨树芽的分支。另外，鉴定了可用于特定植物类型的分子育种的候选基因。我们的结果将提供未来调查杨树分支分子机制的起点。

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