De novo transcriptome assembly and protein profiling of copper-induced lignocellulolytic fungus Ganoderma lucidum MDU-7 reveals genes involved in lignocellulose degradation and terpenoid biosynthetic pathways

Kavish Kumar Jain; Amit Kumar; Akshay Shankar; Dhananjay Pandey; Bhupendra Chaudhary; Krishna Kant Sharma

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De novo transcriptome assembly and protein profiling of copper-induced lignocellulolytic fungus Ganoderma lucidum MDU-7 reveals genes involved in lignocellulose degradation and terpenoid biosynthetic pathways

机译：铜诱导的木质纤维素溶解菌Ganoderma lucidum MDU-7的De Novo转录组合组装和蛋白质分析显示了Ligncellulose降解和Terpenoid生物合成途径所涉及的基因

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Ganoderma lucidum is an important medicinal fungus that possesses exceedingly high lignocellulose degrading ability. Evidently, Cu2+ has decisive roles on the mycelial growth and enzyme production. To reveal the effect of Cu2+ on G. lucidum transcriptome, predominantly associated with lignocellulolytic progression, we conducted comparative NGS based de novo transcriptome assembly using Illumina Hi SeqTM sequencing platform. We obtained 26,083,372 and 35,713,076 high-quality reads from induced and uninduced cultures. For wood degrading activity, 194 transcript coding for oxidoreductases and 402 transcripts for CAZymes were predicted. Further, secretome studies revealed high score GO terms related to oxidoreductases, glycosyl hydrolases, and chitinases from Cu-induced mycelia. The increased Cu2+ concentrations showed higher secretion of lignocellulases such as laccases, cellulases, and xylanases along with increased production of phenolics and antioxidants. Several differences in the transcriptomic and proteomic signatures for lignocellulolytic enzymes provide vital clues about Cu2+ mediated gene regulation and metabolic pathways in basidiomycetous fungi.

机译：灵芝是一种重要的药用真菌，具有极高的木质纤维素降解能力。显然，Cu2 +对菌丝生长和酶生产具有决定性作用。为了揭示Cu2 +对G. lucidum转录组的影响，主要是与木质纤维素溶解进展相关的，我们使用Illumina HI SEQTM测序平台进行了基于Novo转录组组件的比较NGS。我们获得了26,083,372和35,713,076个高质量的诱导和未得到胁迫的培养。对于木材降解活性，预测了194种编码氧化酶的转录物和402种副转录物。此外，沉积研究揭示了与氧化还原酶，糖基水解酶和Cu诱导菌丝菌菌丝的几丁酶相关的高分GO术语。增加的Cu 2 +浓度显示出高曲晶蛋白酶的分泌，例如漆酶，纤维素酶和木聚糖酶以及酚类和抗氧化剂的产生增加。木质纤维素溶解酶的转录组和蛋白质组学特征的几个差异为基础核真菌的Cu2 +介导的基因调控和代谢途径提供了重要的线索。

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《Genomics》 |2020年第1期|共15页
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Kavish Kumar Jain; Amit Kumar; Akshay Shankar; Dhananjay Pandey; Bhupendra Chaudhary; Krishna Kant Sharma;
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Ganoderma lucidumLaccaseNext-generation sequencingProteomeCopper;

机译：Ganoderma lucidumlaccaseNext-Degence SequencingProteMecopper;

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1. De novo transcriptome assembly and protein profiling of copper-induced lignocellulolytic fungus Ganoderma lucidum MDU-7 reveals genes involved in lignocellulose degradation and terpenoid biosynthetic pathways [J] . Jain Kavish Kumar, Kumar Amit, Shankar Akshay, Genomics . 2020,第1期

机译：铜诱导的木质纤维素溶解菌Ganoderma lucidum MDU-7的De Novo转录组合和蛋白质分析显示了涉及木质纤维素降解和萜类生物合成途径的基因
2. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.) [J] . Mohammed Ali, Reem M Hussain, Naveed Ur Rehman, DNA research: an international journal for rapid publication of reports on genes and genomes . 2018,第6期

机译：从头转录组测序和代谢物谱分析表明，复杂的代谢基因参与了蓝茴香（Salvia guaranitica L.）中的萜类生物合成。
3. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.) [J] . Ali Mohammed, Hussain Reem M., Rehman Naveed Ur, DNA research: an international journal for rapid publication of reports on genes and genomes . 2018,第6期

机译：de novo转录组测序和代谢物分析分析显示，在蓝色茴香鼠尾草（Salvia guaranitica l）中涉及三萜类生物合成中涉及的复杂代谢基因。
4. De novo transcriptome sequencing and metabolite profiling analyses reveal the complex metabolic genes involved in the terpenoid biosynthesis in Blue Anise Sage (Salvia guaranitica L.) [O] . Mohammed Ali, Reem M Hussain, Naveed Ur Rehman, 2018

机译：从头转录组测序和代谢物谱分析表明复杂的代谢基因参与了蓝茴香（Salvia guaranitica L.）中的萜类生物合成。
5. De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius) [O] . Lei Zhao, Zhen Liu, Yidi Zhan, 2019

机译：De novo组装和比较转录组分析显示，潜在地参与小麦蚜虫锡中植入饲料的防守萜件排放的基因（Fabricius）

De novo transcriptome assembly and protein profiling of copper-induced lignocellulolytic fungus Ganoderma lucidum MDU-7 reveals genes involved in lignocellulose degradation and terpenoid biosynthetic pathways

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