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首页> 外文期刊>Genomics >Comparative phylogenetic analysis of aquaporins provides insight into the gene family expansion and evolution in plants and their role in drought tolerant and susceptible chickpea cultivars
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Comparative phylogenetic analysis of aquaporins provides insight into the gene family expansion and evolution in plants and their role in drought tolerant and susceptible chickpea cultivars

机译：水蛋白的比较系统发育分析为植物的基因家族扩张和演变提供了深入的洞察力及其在耐旱性和易感鹰嘴豆品种中的作用

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Aquaporins (AQPs) are water channel proteins that play a significant role in drought stress. Although the AQPs identified in multiple plant species, there is no detailed evolutionary and comparative study of AQPs regarding chickpea plant. The current study involved evolutionary analyses coupled with promoter and expression analyses of chickpea AQPs (CaAQPs). A total of 924 non-redundant AQPs were studied in 24 plant species including algae, mosses, lycophytes, monocots and dicots. Phylogenetic analysis demonstrated a clear divergence of eight AQP subfamilies (LIPs, SIPs, GIPs, NIPs, XIPs, PIPs, HIPs and TIPs). The comparative phylogenetic trees of AQP subfamilies among Arabidopsis, soybean, common bean, maize and chickpea demonstrated that the AQPs were highly species-specific. Interestingly, the dual NPA motif was conserved in all species. However, the ar/R selectivity filter signatures [W/T/S/N/G/A]-[V/S/L/I/A]-[S/G/A]-R (in NIPs), F-H-T-R (in PIPs), [H/N/Q/S]-[A/I/L/S/V]-[A/G]-[A/C/L/M/R/V] (in TIPs) and [V/I/L/M]-[V/I/A/F/M]-[A/S/F/C]-[N/F/L/I/A/S (in SIPs) were found in five species. Moreover, the Frogers positions (P1-P5) were found as [F/L/Y]-[S/T]-A-Y-[L/I/M/V/F] (in NIPs), [Q/E/M]-S-A-F-W (in PIPs), [A/L/S/T/V]-[A/C/N/S/T/V]-[P/R/S]-[Y/N/F]-[W/Q] (in TIPs) and [I/M/F]-[A/V]-[A/V]-Y-W (in SIPs). The MEME motif analyses showed that most of the motifs were specific to subfamily and subgroups. Tissue-specific expression profiling of CaAQPs revealed that CaTIPs and CaPIPs are highly expressed in most of the tissues, while CaNIPs and CaSIPs have low expression. In promoter analysis of CaAQPs, multiple stress-related cis-acting elements e.g. MYB, MYC, ABRE, etc. were found. Semi-quantitative RT-PCR analysis showed that CaPIP2;3 and CaNIP3;1 are positive regulator, while CaSIP1;1 and CaPIP2;1 have a negative role in drought tolerance. The findings and implications of this study are discussed in detail.
机译：Aquaporins（AQPS）是在干旱胁迫下发挥重要作用的水通道蛋白。虽然在多种植物物种中鉴定的AQP，但没有关于鹰嘴豆植物AQP的详细进化和比较研究。目前的研究涉及进化分析与鹰嘴豆（CAAQPS）的启动子和表达分析偶联。在包括藻类，苔藓，溶菌酶，单焦质和双胞胎的24种植物物种中，共有924种非冗余AQP。系统发育分析表明，八个AQP亚属（嘴唇，嘴唇，咯，NIPS，XIPS，PIPS，HIPS和提示）的透明分歧。 AQP亚麻籽，大豆，常见豆类，玉米和鹰嘴豆中AQP亚毛利的比较系统发育树证明了AQP是高度特异性的。有趣的是，双NPA主题在所有物种中被保守。但是，AR / R选择性滤波器签名[w / t / s / n / g / a] - [v / s / l / i / a] - [s / g / a] -r（nips），fhtr （在PIPS中），[H / N / Q / S] - [A / I / L / S / V] - [A / G] - [A / C / L / M / R / V]（提示） [v / i / l / m] - [v / i / a / f / m] - [a / s / f / c] - [n / f / l / i / a / s（在啜饮中）是发现五种物种。此外，发现蛙人的位置（P1-P5）被发现为[F / L / Y] - [S / T] - [L / I / M / V / F]（在NIPS中），[Q / E / m] -safw（pips），[a / l / s / t / v] - [a / c / n / s / t / v] - [p / r / s] - [y / n / f] - [w / q]（提示）和[I / m / f] - [a / v] - [a / v] -yw（啜饮）。 MEME MOTIF分析表明，大多数基序是亚家族和亚组的特异性。 CAAQP的组织特异性表达分析显示，在大多数组织中，曲线和曲面高度表达，而Canips和CaseIPS表达低。在CAAQPS的启动子分析中，多重应力相关的顺式作用元素例如。发现了MYB，MYC，ABRE等。半定量RT-PCR分析显示Capip2; 3和Canip3; 1是正调节器，而Casip1; 1和Capip2; 1在旱润耐受中具有负面作用。详细讨论了该研究的发现和含义。

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