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首页> 外文期刊>Genomics >Systematic study of the stress-responsive Rboh gene family in Nicotiana tabacum: Genome-wide identification, evolution and role in disease resistance
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Systematic study of the stress-responsive Rboh gene family in Nicotiana tabacum: Genome-wide identification, evolution and role in disease resistance

机译：尼加托氏菌镇应力响应RBOH基因家族的系统研究：基因组鉴定，进化和抗病性作用

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Plant respiratory burst oxidase homolog (Rboh) gene family encodes the key enzymatic subunits of reactive oxygen species (ROS) production pathways, and play crucial role in plant signaling, development and stress responses. In present work, twenty genes were identified in Nicotiana tabacum Rboh family (NtabRboh) and classified into four phylogenetic groups (I-IV). Fourteen NtabRboh genes were positioned on ten chromosomes (i.e., Ch1, 2, 4, 7–11, 14 and 21), and six scaffolds. Synteny and evolutionary analysis showed that most of the NtabRboh genes have evolved from the genomes of the ancestor species (N.?tomentosiformis and N.?sylvestris), which afterwards expanded through duplication events. The promoter regions of the NtabRboh genes contained numerous cis-acting regulatory elements for hormones, plant growth, and different biotic and abiotic factors. The NtabRbohF gene transcript comprised target sites for wounding and stress responsive microRNAs: nta-miR166a-d, g and h. The transcript abundance of NtabRboh genes in different tissues reflected their important for plant growth and organ development in tobacco. RT-qPCR-assays demonstrated that the expression of NtabRboh genes are regulated by viral and bacterial pathogens, drought, cold and cadmium stress. The expression levels NtabRbohA, B and C were significantly up-regulated in “black shank and tobacco mosaic virus-inoculated susceptible and transgenic tobacco cultivars, showing that these genes play important roles in disease resistance.
机译：植物呼吸爆发氧化酶同源物（RBOH）基因家族编码反应性氧物质（ROS）生产途径的关键酶酶，并在植物信号传导，发育和应力反应中发挥至关重要的作用。在目前的工作中，在尼古特塔巴氏菌族（Ntabrboh）中鉴定出二十个基因，并分为四个系统发育基团（I-IV）。 14个NTABRBOH基因定位在十染色体上（即CH1,2,4,7-11,14和21）和六个支架。 Synteny和进化分析表明，大多数NTABRBOH基因已经从祖先物种的基因组（N.Ontomentosiformis和N.? ylvestris）演变，之后通过复制事件扩增。 NTABRBOH基因的启动子区含有许多用于激素，植物生长和不同生物和非生物因子的CIS作用调节元件。 NTABRBOHF基因转录物包含用于伤害和应激响应微大罗氏的靶位点：NTA-MIR166A-D，G和H。不同组织中NTABRBOH基因的成绩丰度反映了烟草中植物生长和器官发育的重要性。 RT-QPCR测定证明，NTABRBOH基因的表达由病毒和细菌病原体，干旱，冷和镉胁迫调节。表达水平Ntabrboha，B和C在“黑色柄和烟草叶片病毒接种的易感和转基因烟草品种中显着上调，表明这些基因在抗病性中起重要作用。

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