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首页> 外文期刊>Genomics >Chloroplast genome sequences of Artemisia maritima and Artemisia absinthium: Comparative analyses, mutational hotspots in genus Artemisia and phylogeny in family Asteraceae
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Chloroplast genome sequences of Artemisia maritima and Artemisia absinthium: Comparative analyses, mutational hotspots in genus Artemisia and phylogeny in family Asteraceae

机译：Artemisia Maritima和Artemisia Absintehium的叶绿体基因组序列：比较分析，艾蒿属的突变热点和菊属植物的系统

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摘要

Artemisia L. is a complex genus of medicinal importance. Publicly available chloroplast genomes of few Artemisia species are insufficient to resolve taxonomic discrepancies at species level. We report chloroplast genome sequences of two further Artemisia species: A. maritima (151,061?bp) and A. absinthium (151,193?bp). Both genomes possess typical quadripartite structure comprising of a large single copy, a small single copy and a pair of long inverted repeats. The two genomes exhibited high similarities in genome sizes, gene synteny, GC content, synonymous and non-synonymous substitutions, codon usage, amino acids frequencies, RNA editing sites, microsatellites, and oligonucleotide repeats. Transition to transversion ratio was 1. Maximum likelihood tree showed Artemisia a monophyletic genus, sister to genus Chrysanthemum. We also identified 20 highly polymorphic regions including rpoC2-rps2, trnR-UCU-trnG-UCC, rps18-rpl20, and trnL-UAG-rpl32 that could be used to develop authentic and cost-effective markers to resolve taxonomic discrepancies and infer phylogenetic relationships among Artemisia species.
机译：Artemisia L.是药用重要性的复杂属。少数蒿属植物的公开可获得的叶绿体基因组不足以解决物种水平的分类学差异。我们报告两种进一步的蒿属植物的叶绿体基因组序列：A.Maritima（151,061〜BP）和A.缺苗（151,193？BP）。两个基因组具有典型的四脚石结构，包括大单拷贝，小单拷贝和一对长反转重复。这两个基因组在基因组大小，基因同联，GC含量，同义和非同义级取代，密码子使用，氨基酸频率，RNA编辑位点，微卫星和寡核苷酸重复中的高相似之处。过渡到颠转率为<1。最大可能性树显示蒿属植物，菊属的姐妹。我们还确定了20个高度多态性区域，包括RPOC2-RPS2，TRNR-UCU-TRNG-UCC，RPS18-RPL20和TRNL-UAG-RPL32，可用于开发正宗和经济有效的标记以解决分类学差异和推断系统发育关系在蒿属中。

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