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首页> 外文期刊>Genomics >Tissue-specific transcriptome for Dendrobium officinale reveals genes involved in flavonoid biosynthesis
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Tissue-specific transcriptome for Dendrobium officinale reveals genes involved in flavonoid biosynthesis

机译：Dendrobium Officinale的组织特异性转录组揭示了类黄酮生物合成所涉及的基因

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摘要

Dendrobium officinale is a kind of crop and precious herbal that is widely distributed in China. We applied transcriptomics to investigate the flavonoids and their biosynthesis-related genes from different tissues. Total flavonoid was determined in three different tissues. In this study, nine cDNA libraries were generated from Dendrobium officinale. A total of 530 million (70.73%) of the high-quality reads were successfully mapped to the reference genome of Dendrobium officinale and nine libraries were combined and assembled into 24,927 non-redundant genes. We mapped all of these genes to reference pathways in the KEGG database to identify polysaccharide and secondary metabolites pathways in which the genes may be involved. We outlined the biosynthetic pathway of flavonoids and identified putative genes, which provide understanding of the biosynthesis and regulation of Dendrobium officinale flavonoids at the molecular level. We identified five CHS genes from Dendrobium officinale and characterized the CHS gene family. The flavonoid-related key enzyme genes were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR. These data on flavonoid genes obtained in this work will be useful in understanding the molecular mechanisms of different tissues in Dendrobium officinale.
机译：Dendrobium Officinale是一种在中国广泛分布的作物和珍贵的草药。我们应用转录组织从不同组织中探讨了类黄酮及其生物合成相关基因。在三种不同的组织中测定总类黄酮。在这项研究中，从石斛属officinale产生了九个cDNA文库。总共5.3亿（70.73％）的高质量读数成功地映射到石斛officinale的参考基因组，并将九个文库组合成24,927个非冗余基因。我们将所有这些基因映射到Kegg数据库中的参考途径，以鉴定多糖和次生代谢物途径，其中可能涉及基因。我们概述了黄酮类化合物的生物合成途径和鉴定的诱发基因，其在分子水平下提供了对生物合成和石斛中石斛黄酮类化合物的理解。我们鉴定了来自Dendrobium Officinale的五个CHS基因，并表征了CHS基因家族。鉴定了类黄酮相关的关键酶基因，使用定量实时PCR进一步分析不同组织中的表达模式。在本工作中获得的类黄酮基因的这些数据对于了解Dendrobium Officinale中不同组织的分子机制。

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