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首页> 外文期刊>Genomics >Comparative genomics of a subset of Adherent/Invasive Escherichia coli strains isolated from individuals without inflammatory bowel disease
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Comparative genomics of a subset of Adherent/Invasive Escherichia coli strains isolated from individuals without inflammatory bowel disease

机译：没有炎症肠病的个体粘附/侵袭性大肠杆菌菌株子集的比较基因组学

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There is increased evidence demonstrating the association between Crohns Disease (CD), a type of Inflammatory Bowel Disease (IBD), and non-diarrheagenic Adherent/Invasive Escherichia coli (AIEC) isolates. AIEC strains are phenotypically characterized by their adhesion, invasion and intra-macrophage survival capabilities. In the present study, the genomes of five AIEC strains isolated from individuals without IBD (four from healthy donors and one from peritoneal liquid) were sequenced and compared with AIEC prototype strains (LF82 and NRG857c), and with extra-intestinal uropathogenic strain (UPEC CFT073). Non-IBD-AIEC strains showed an Average Nucleotide Identity up to 98% compared with control strains. Blast identities of the five non-IBD-AIEC strains were higher when compared to AIEC and UPEC reference strains than with another E. coli pathotypes, suggesting a relationship between them. The SNPs phylogeny grouped the five non-IBD-AIEC strains in one separated cluster, which indicates the emergence of these strains apart from the AIEC group. Additionally, four genomic islands not previously reported in AIEC strains were identified. An incomplete Type VI secretion system was found in non-IBD-AIEC strains; however, the Type II secretion system was complete. Several groups of genes reported in AIEC strains were searched in the five non-IBD-AIEC strains, and the presence of fimA, fliC, fuhD, chuA, irp2 and cvaC were confirmed. Other virulence factors were detected in non-IBD-AIEC strains, which were absent in AIEC reference strains, including EhaG, non-fimbrial adhesin 1, PapG, F17D-G, YehA/D, FeuC, IucD, CbtA, VgrG-1, Cnf1 and HlyE. Based on the differences in virulence determinants and SNPs, it is plausible to suggest that non-IBD AIEC strains belong to a different pathotype.
机译：增加了证据证明克罗斯病（CD），一种炎性肠病（IBD）和非腹泻的粘附/侵袭性大肠杆菌（AIEC）分离物之间的证据。 AIEC菌株的特征在于它们的粘附，侵袭和巨噬细胞存活能力。在本研究中，测序从没有IBD的个体中分离的五个AIEC菌株的基因组（来自健康供体中的四个来自腹膜液），与AIEC原型菌株（LF82和NRG857C）进行比较，并具有外肠道尿血糖菌株（UPEC CFT073）。与对照菌株相比，非IBD-AIEC菌株显示出高达98％的平均核苷酸同一性。与AIEC和UPEC参考菌株相比，五个非IBD-AIEC菌株的爆破标识比其他大肠杆菌病理型更高，表明它们之间的关系。 SNPS phylogeny在一个分离的簇中将五个非IBD-AIEC菌株分组，这表明与AIEC组相比这些菌株的出现。另外，鉴定了先前未报告的四种基因组岛。在非IBD-AIEC菌株中发现了不完全的VI分泌系统;但是，II型分泌系统完成。在五个非IBD-AIEC菌株中搜索了在AIEC菌株中报道的几组基因，并确认了FIMA，FLIC，FUHD，CHUA，IRP2和CVAC的存在。在非IBD-AIEC菌株中检测到其他毒力因子，其在AIEC参考菌株中不存在，包括EHAG，非绒毛粘蛋白1，PAPG，F17D-G，YEHA / D，CAUC，IUCD，CBTA，VGRG-1， cnf1和hlye。基于毒力决定簇和SNP的差异，表明非IBD AIEC菌株属于不同的病例是合理的。

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