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首页> 外文期刊>Genomics >Function and characterization of the promoter region of perilipin 1 (PLIN1): Roles of E2F1, PLAG1, C/EBPβ, and SMAD3 in bovine adipocytes
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Function and characterization of the promoter region of perilipin 1 (PLIN1): Roles of E2F1, PLAG1, C/EBPβ, and SMAD3 in bovine adipocytes

机译：Perilipin 1（PLIN1）启动子区域的功能和表征：E2F1，PLAG1，C /EBPβ和SMAD3在牛脂肪细胞中的作用

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摘要

Perilipin 1 (PLIN1) protein, also known as lipid droplet-associated protein, is encoded by the PLIN1 gene and is able to anchor itself to the membranes of lipid droplets. The phosphorylation of PLIN1 is critical for the mobilization of fat in adipose tissue and plays an important role in regulating lipolysis and lipid storage in adipocytes. However, research on the synthesis and lipid metabolism of lipid droplets by PLIN1 in bovine adipocytes is limited. In the present study, we found that bovine PLIN1 was highly expressed in subcutaneous adipose tissue. The highest level of PLIN1 mRNA expression in bovine adipocytes was observed on day 6 of differentiation. Moreover, the cytoplasmic subcellular localization of PLIN1 was observed in bovine preadipocytes. To elucidate the molecular mechanism of bovine PLIN1 transcriptional regulation, we cloned eight fragments containing the 5′ regulatory region of the PLIN1 gene. The results showed that the ?209/?17?bp region of the bovine PLIN1 gene was the core promoter region. Based on the transcriptional activities of the promoter vector fragments, the luciferase activity of the mutated fragment, the siRNA interference, and the results of the electrophoretic mobility shift assay (EMSA), we identified the binding sites of E2F transcription factor 1 (E2F1), pleiomorphic adenoma gene 1 (PLAG1), CCAAT enhancer binding protein beta (C/EBPβ), and SMAD family member 3 (SMAD3) as the transcriptional activators or repressors of the core promoter region. Further experiments confirmed that the knockdown of the PLIN1 gene affected the ability of these transcription factors to regulate the lipid metabolism in bovine adipocytes. In conclusion, our results reveal a potential mechanism for the transcriptional regulation of PLIN1 in bovine adipocytes.
机译：Perilipin 1（plin1）蛋白，也称为脂滴相关蛋白，由PLIN1基因编码，并且能够将其自身锚固到脂质滴的膜上。 PLIN1的磷酸化对于动员脂肪组织中脂肪的动员至关重要，并在调节脂肪细胞中调节脂解和脂质储存的重要作用。然而，PLIN1在牛脂肪细胞中通过PLIN1的合成和脂质代谢的研究受到限制。在本研究中，我们发现牛Plin1在皮下脂肪组织中高度表达。在分化的第6天观察到牛脂肪细胞中的最高水平的牛脂肪细胞表达。此外，在牛前脂肪细胞中观察到PLIN1的细胞质亚细胞定位。为了阐明牛Plin1转录调节的分子机制，我们克隆了含有PLIN1基因的5'调节区的八个片段。结果表明，牛PLIN1基因的α209/α17Δ17≤17μla。核心启动子区。基于启动子载体片段的转录活性，突变片段的荧光素酶活性，siRNA干扰和电泳迁移率移位测定（EMSA）的结果，我们鉴定了E2F转录因子1（E2F1）的结合位点，肺炎腺瘤基因1（PLAG1），CCAAT增强剂结合蛋白β（C /EBPβ），以及Smad家族构件3（SMAD3）作为核心启动子区域的转录活化剂或阻遏物。进一步的实验证实，PLIN1基因的敲低影响了这些转录因子调节牛脂肪细胞中脂质代谢的能力。总之，我们的结果揭示了牛脂肪细胞中PLIN1转录调控的潜在机制。

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