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首页> 外文期刊>Genomics >Identification and characterization of differentially expressed genes in the rice root following exogenous application of spermidine during salt stress
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Identification and characterization of differentially expressed genes in the rice root following exogenous application of spermidine during salt stress

机译：盐胁迫期间亚源性外源施用稻根系差异表达基因的鉴定与表征

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Salinity is a major limiting factor in crop production. Exogenous spermidine (spd) effectively ameliorates salt injury, though the underlying molecular mechanism is poorly understood. We have used a suppression subtractive hybridization method to construct a cDNA library that has identified up-regulated genes from rice root under the treatment of spd and salt. Total 175 high-quality ESTs of about 100–500?bp in length with an average size of 200?bp are isolated, clustered and assembled into a collection of 62 unigenes. Gene ontology analysis using the KEGG pathway annotation database has classified the unigenes into 5 main functional categories and 13 subcategories. The transcripts abundance has been validated using Real-Time PCR. We have observed seven different types of post-translational modifications in the DEPs. 44 transmembrane helixes are predicted in 6 DEPs. This above information can be used as first-hand data for dissecting the administrative role of spd during salinity.
机译：盐度是作物生产中的主要限制因素。外源性山晶体（SPD）有效改善盐损伤，但潜在的分子机制理解得很差。我们使用了抑制消减杂交方法来构建CDNA文库，其在SPD和盐的治疗下从水稻根系中鉴定了上调基因。总共175个高质量的大约100-500？BP的长度为200？BP的平均大小，聚集在一起并组装成62个unigenes的集合。使用Kegg Pathway注释数据库将未成语分类为5个主要功能类别和13个子类别的基因本体分析。通过实时PCR验证了转录物丰富。我们在DEPS中观察到七种不同类型的翻译后修改。 44透气螺旋在6张中预测。以上信息可以用作解剖SPD在盐度期间SPD的行政作用的第一手数据。

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