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首页> 外文期刊>Global change biology bioenergy >Synthetic polyploid production of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus
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Synthetic polyploid production of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus

机译：Miscanthus sacchariflorus，miscanthus sinensis和miscanthus x giganteus的合成多倍体生产

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Plants from the genus Miscanthus are potential renewable sources of lignocellulosic biomass for energy production. A potential strategy for Miscanthus crop improvement involves interspecific manipulation of ploidy levels to generate superior germplasm and to circumvent reproductive barriers for the introduction of new genetic variation into core germplasm. Synthetic autotetraploid lines of Miscanthus sacchariflorus and Miscanthus sinensis, and autoallohexaploid Miscanthus x giganteus were produced in tissue culture from oryzalin treatments to seed- and immature inflorescence-derived callus lines. This is the first report of the genome doubling of diploid M. sacchariflorus. Genome doubling of diploid M. sinensis, M. sacchariflorus, and triploid M. x giganteus to generate tetraploid and hexaploid lines was confirmed by stomata size, nuclear DNA content, and chromosome counts. A putative pentaploid line was also identified among the M. x giganteus synthetic polyploid lines by nuclear DNA content and chromosome counts. Comparisons of phenotypic performance of synthetic polyploid lines with their diploid and triploid progenitors in the greenhouse found species-specific differences in plant tiller number, height, and flowering time among the doubled lines. Stem diameter tended to increase after polyploidization but there were no significant improvements in biomass traits. Under field conditions, M. x giganteus synthetic hexaploid lines showed greater phenotypic variation, in terms of plant height, stem diameter, and tiller number, than their progenitor lines. Production of synthetic autopolyploid lines displaying significant phenotypic variation suggests that ploidy manipulation can introduce useful genetic diversity in the limited Miscanthus germplasm currently available in the United States. The role of polyploidization in the evolution and breeding of the genus Miscanthus is discussed.
机译：来自Miscanthus属的植物是能源生产的木质纤维素生物量的潜在可再生能源。 Miscanthus作物改善的潜在策略涉及倍增性水平的特异性操纵，以产生优异的种质，并使生殖屏障引入核心种质中的新遗传变异。 Miscanthus Sacchariflorus和Miscanthus sinensis的合成自身传递线和Miscanthus Sincanthus X Giganteus在组织培养物中，从橘子素治疗中的组织培养物中产生种子和未成熟的花序衍生的愈伤组织。这是二倍体M. Sacchariflorus的基因组倍增的第一个报告。通过气孔大小，核DNA含量和染色体计数证实了二倍体M. Sinensis，M.Sacchariflorus和三倍体M.X Giganteus的基因组。通过核DNA含量和染色体计数，还鉴定了一种推定的五萜倍细系。核DNA含量和染色体计数。在温室中与二倍体和三倍体祖细胞的合成多倍体系表型性能的比较发现了植物分蘖数，高度和翻转时间的种类特异性差异。茎直径倾向于多倍化后增加，但生物质性状没有显着改善。在现场条件下，M。X Giganteus合成六倍线在植物高度，茎直径和分蘖数方面表现出更大的表型变异，而不是其祖细胞系。展示显着的表型变异的合成自电节管线的生产表明，倍性操纵可以在当前在美国目前可用的有限MISCanthus种质中引入有用的遗传多样性。讨论了多倍化在Miscanthus的演变和育种中的作用。

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