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DNA barcoding: A practical tool for the taxonomy and species identification of entomofauna

机译：DNA条形码：昆虫的分类和物种鉴定的实用工具

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DNA barcoding is an innovative system designed to provide rapid, accurate, and automatable species identification by using short, standardized gene regions as internal species codes. The mitochondrial cytochrome C oxidase I (COI) gene was proposed by Paul Hebert as an official marker for animals, because of its small intraspecific but large interspecific variation. Since the launch of the project Barcode of Life, this simple technique has caught the interest of taxonomists, ecologists and plant-quarantine officers charged with the control of pests and invasive species. The great diversity of insects and their importance have made this group a major target for DNA barcoding. In most cases, the identification of insect species by traditional methods based on morphological features requires specialist knowledge and is labor-intensive. DNA barcoding aims at meeting the challenge of monitoring and documenting the biodiversity of insects. The utility of DNA barcoding for identifying small insects, cryptic taxa or rare species, as well as many species of forest entomofauna that are impossible to discriminate morphologically throughout all of their life stages, is a subject discussed in this review. Due to its usefulness, also in Poland in the Forestry Research Institute, a method for identifying selected species of saproxylic beetles based on the sequence of the COI region was developed. In the future, this method will be used to assess the state of biodiversity and the naturalness of forest ecosystems. Therefore, this and other future implications of this promising new technique are also discussed here.
机译：DNA条形码是一种创新系统，旨在通过使用短，标准化的基因区域作为内部物种代码提供快速，准确和自动的物种鉴定。 Paul Hebert作为动物的官方标记提出了线粒体细胞色素C氧化酶I（COI）基因，因为它的内部内含性小，但大的间隙差异很大。自从生命的项目条形码推出以来，这种简单的技术抓住了分类师，生态学家和植物检疫人员被控控制害虫和侵入物种的兴趣。昆虫的巨大多样性及其重要性使该组成为DNA条形码的主要目标。在大多数情况下，通过基于形态学特征的传统方法鉴定昆虫物种需要专业知识，并且是劳动密集型的。 DNA条形码旨在满足监测和记录昆虫生物多样性的挑战。 DNA条形码用于鉴定小昆虫，隐秘的分类群或稀有物种的效用以及许多不可能在其所有生命阶段中歧视形态的许多森林昆虫昆虫，是本次审查中讨论的主题。由于其有用性，也是在林业研究所的波兰，开发了一种基于COI区序列鉴定所选择的皂甲虫甲虫的方法。将来，这种方法将用于评估生物多样性和森林生态系统的自然状态。因此，这里还讨论了这一有前途的新技术的这种和其他未来的影响。

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