...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Microbiology >Comparative Genomic Analysis of a Clinical Isolate of Klebsiella quasipneumoniae subsp. similipneumoniae, a KPC-2 and OKP-B-6 Beta-Lactamases Producer Harboring Two Drug-Resistance Plasmids from Southeast Brazil
【24h】

Comparative Genomic Analysis of a Clinical Isolate of Klebsiella quasipneumoniae subsp. similipneumoniae, a KPC-2 and OKP-B-6 Beta-Lactamases Producer Harboring Two Drug-Resistance Plasmids from Southeast Brazil

机译：<斜视> klebsiella QuaSipneumoniae 亚木临床分离物的比较基因组分析。 <斜视> Similipneumoniae ，KPC-2和OKP-B-6β-内酰胺制片人从巴西东南部携带两种耐药质粒

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to unravel the genetic determinants responsible for multidrug (including carbapenems) resistance and virulence in a clinical isolate of Klebsiella quasipneumoniae subsp. similipneumoniae by whole-genome sequencing and comparative analyses. Eighty-three clinical isolates initially identified as carbapenem-resistant K. pneumoniae were collected from nosocomial infections in southeast Brazil. After RAPD screening, the KPC-142 isolate, showing the most divergent DNA pattern, was selected for complete genome sequencing in an Illumina HiSeq 2500 instrument. Reads were assembled into scaffolds, gaps between scaffolds were resolved by in silico gap filling and extensive bioinformatics analyses were performed, using multiple comparative analysis tools and databases. Genome sequencing allowed to correct the classification of the KPC-142 isolate as K. quasipneumoniae subsp. similipneumoniae . To the best of our knowledge this is the first complete genome reported to date of a clinical isolate of this subspecies harboring both class A beta-lactamases KPC-2 and OKP-B-6 from South America. KPC-142 has one 5.2 Mbp chromosome (57.8% G+C) and two plasmids: 190 Kbp p KQPS142a (50.7% G+C) and 11 Kbp p KQPS142b (57.3% G+C). The 3 Kbp region in p KQPS142b containing the bla _(KPC?2)was found highly similar to that of p Kp13d of K. pneumoniae Kp13 isolated in Southern Brazil in 2009, suggesting the horizontal transfer of this resistance gene between different species of Klebsiella . KPC-142 additionally harbors an integrative conjugative element ICE Pm1 that could be involved in the mobilization of p KQPS142b and determinants of resistance to other classes of antimicrobials, including aminoglycoside and silver. We present the completely assembled genome sequence of a clinical isolate of K. quasipneumoniae subsp. similipneumoniae , a KPC-2 and OKP-B-6 beta-lactamases producer and discuss the most relevant genomic features of this important resistant pathogen in comparison to several strains belonging to K. quasipneumoniae subsp. similipneumoniae (phylogroup II-B), K. quasipneumoniae subsp. quasipneumoniae (phylogroup II-A), K. pneumoniae (phylogroup I), and K. variicola (phylogroup III). Our study contributes to the description of the characteristics of a novel K. quasipneumoniae subsp. similipneumoniae strain circulating in South America that currently represent a serious potential risk for nosocomial settings.
机译：本研究的目的是在临床中孤立的喹硫蛋白亚皮中，揭开负责多药（包括碳癌蛋白）抗性和毒力的遗传决定因素。 Similipneumoniae通过全基因组测序和比较分析。最初鉴定为CarbapeNem抗性K.肺炎的八十三个临床分离物被从东南巴西的医院感染收集。在RAPD筛选后，选择kPC-142分离物，显示最分散的DNA模式，用于在Illumina Hiseq 2500仪器中完全基因组测序。将读数组装成支架，通过使用多个比较分析工具和数据库进行硅间隙填充和广泛的生物信息学分析。基因组测序允许校正KPC-142分离物的分类为K. QuAsipneumoniae亚空间。 Similipneumoniae。据我们所知，这是第一个完整的基因组，迄今为止迄今为止患有南美洲的β-内酰胺kpc-2和okp-b-6的临床孤立的临床孤立。 KPC-142具有5.2Mbp染色体（57.8％G + C）和两种质粒：190kbp p kqps142a（50.7％g + c）和11kbp p kqps142b（57.3％g + c）。含有BLA _（KPCα2）的P kQPS142b中的3kbp区域非常类似于2009年巴西南部南部患者的Kp13d的P kp13d的高度相似，这表明这种抗性基因之间的水平转移了不同种类的克利布拉之间。 KPC-142另外，含有可共同的共轭元素冰PM1，其可参与动员P kQPS142B和抗性抗菌剂的抗性的决定因素，包括氨基糖苷和银。我们介绍了K.QuaSipneumoniae患者的临床分离物的完全组装的基因组序列。 Similipneumoniae，KPC-2和OKP-B-6β-内酰胺酶生产商，并与属于K. QuAsipneumoniae Subsp的几种菌株相比，探讨了这种重要抗性病原体的最相关的基因组特征。 Similipnneumoniae（Phylogroup II-B），K. Quasipneumoniae亚普。 QuaSipneumoniae（Phylogroup II-A），K.Pneumoniae（Phylogroup I）和K.Variicola（Phylogroup III）。我们的研究有助于描述新型K. QuAsipneumoniae亚普的特征。在南美洲循环的Similipneumoniae菌株目前代表了医院环境的严重潜在风险。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号