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首页> 外文期刊>Frontiers in Immunology >The Significance of Discordant Serology in Chagas Disease: Enhanced T-Cell Immunity to Trypanosoma cruzi in Serodiscordant Subjects
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The Significance of Discordant Serology in Chagas Disease: Enhanced T-Cell Immunity to Trypanosoma cruzi in Serodiscordant Subjects

机译：不间生素血管疾病中不间断的血清学的意义：增强T细胞免疫<斜视>锥虫瘤Cruzi

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Background Subjects are considered infected with Trypanosoma cruzi when tested positive by at least two out of three serological tests, whereas a positive result in only one of up to three tests is termed “serodiscordant” (SD). Assessment of parasite-specific T-cell responses may help discriminate the uninfected from infected individuals among SD subjects. Methods Peripheral blood mononuclear cells from SD and seropositive (SP) subjects, who were born in areas endemic for T. cruzi infection but living in Buenos Aires city, Argentina, at the time of the study, and seronegative unexposed subjects were included for analysis. The function and phenotype of T cells were assessed by interferon-γ (IFN-γ) and interleukin (IL)-2 enzyme-linked immunospot assay and multiparameter flow cytometry. T. cruzi -specific antibodies were quantified by conventional serology and a multiplex assay format. Results SD subjects exhibited immunity cell responses to T. cruzi but in contrast to SP subjects, T cells in SD subjects more often display the simultaneous production of IFN-γ and IL-2 in response to T. cruzi antigens and have a resting phenotype. SD individuals also have higher IFN-γ spot counts, polyfunctional CD4~(+)T-cells enriched in IL-2 secreting cells and low levels of antibodies specific for a set of T. cruzi -derived recombinant proteins compared with the SP group. Long-term follow-up of SD individuals confirmed that humoral and T-cell responses fluctuate but are sustained over time in these subjects. T cells in SD subjects for T. cruzi infection did not recognize Leishmania antigens. Conclusion Both T-cell and humoral responses in most subjects assessed by conventional tests as SD for T. cruzi infection indicate prior exposure to infection and the establishment of immunological memory suggestive of a resolved infection.
机译：当通过三种血清学试验中至少有两种血清学测试测试阳性时，被认为受试者感染了锥虫瘤瘤，而最多三种测试中的一个阳性结果被称为“SERODASCOSCORDANT”（SD）。寄生虫特异性T细胞应答的评估可能有助于区分从SD受试者中的受感染的个体的未感染。方法采用SD和血清阳性（SP）受试者的外周血单核细胞，他出生于特有的T.Cruzi感染，但居住在阿根廷的Buenos Aires City，在研究中，包括血清可未暴露的受试者进行分析。通过干扰素-γ（IFN-γ）和白细胞介素（IL）-2酶连接的免疫表特测定和多脉络流量细胞仪评估T细胞的功能和表型。通过常规血清学和多重测定形式量化克鲁齐氏抗体。结果SD受试者表现出对T.Cruzi的免疫细胞反应，但与SP受试者相比，SD受试者的T细胞更常常显示IFN-γ和IL-2的同时产生IFN-γ和IL-2的响应于T.Cruzi抗原并具有静止表型。 SD个体还具有更高的IFN-γ斑点计数，富含IL-2分泌细胞的多官能CD4〜（+）T细胞，以及与SP组相比，对一组T.Cruzi - 一定的重组蛋白特异的低水平抗体。 SD个人的长期随访证实了液体和T细胞应对波动但在这些受试者中随着时间的推移。 SD受试者中的T细胞T.Cruzi感染不承认Leishmania抗原。结论常规试验评估的大多数受试者中的T细胞和体液反应均为克鲁齐感染表明接触感染和建立分辨感染的免疫记忆的建立。

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